Composição química e atividade antimicrobiana do óleo essencial e anatomia foliar e caulinar de Citrus limettioides Tanaka (Rutaceae)
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Data
2013
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Resumo
Citrus limettioides (Rutaceae), a sweet lime, is popularly
used to treat sinusitis, and as an antipyretic and
hypotensive agent. The aim of this study was to identify
the constituents of the essential oils from the leaf and
fruit peel of C. limettioides, test the antimicrobial activity
of these essential oils and perform an anatomical study
of the leaf and young stem. Essential oils were isolated
by steam distillation in a modified Clevenger apparatus
and analyzed by GC-MS. The minimum inhibitory
concentrations (MIC) against Gram-positive and Gramnegative
bacteria and fungi were determined by broth
microdilution. Anatomical studies were performed
on freehand histological sections, which were stained
with Alcian blue / safranin, Steinmetz reagent and
lugol and analyzed by light microscopy. Linalol was
the major component in the leaf essential oil (36.88
to 45.15%) and limonene in the fruit peel essential oil
(70.60 to 75.18%). The essential oil from leaves showed
antimicrobial activity against bacteria (MIC 2000 μg/
mL) and no activity against fungi. The essential oil from
the fruit peel showed no antimicrobial activity. The
leaf is hypostomatic with predominance of anomocytic
stomata, the mesophyll is dorsiventral with biseriate
palisade and 11 layers of spongy parenchyma cells.
Secretory cavities were observed among the mesophyll
cells of the leaf, petiole and stem, whilst prismatic
crystals were identified in all structures of leaf and
stem. The anatomical data obtained in the microscopic
study may help in the taxonomic identification of this
plant and to verify the authenticity of the drug plant.
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Palavras-chave
Citrus limettioides, Estudo anatômico, Óleos essenciais, Controle de qualidade, Atividade antimicrobiana, Citrus limettioides, Anatomical study, Essential oils, Quality control, Antimicrobial activity
Citação
LOPES, Larissa Teodoro Alves et al. Composição química e atividade antimicrobiana do óleo essencial e anatomia foliar e caulinar de Citrus limettioides Tanaka (Rutaceae). Revista de Ciências Farmacêuticas Básica e Aplicada, Araraquara, v. 34, n. 4, p. 503-511, 2013.