Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/16567
Tipo do documento: Artigo
Título: Immunogenicity of a fusion protein containing immunodominant epitopes of Ag85C, MPT51, and HspX from Mycobacterium tuberculosis in mice and active TB infection
Autor: Sousa, Eduardo Martins de
Costa, Adeliane Castro da
Trentini, Monalisa Martins
Araújo Filho, João Alves de
Kipnis, André
Junqueira-Kipnis, Ana Paula
Abstract: Tuberculosis (TB) remains a major global health problem. The only vaccine against tuberculosis, attenuated Mycobacterium bovis Bacillus Calmette-Guerin (BCG), has demonstrated relatively low efficacy and does not provide satisfactory protection against the disease in adults. More effective vaccines and better therapies are urgently needed to reduce the global spread of TB. This study evaluated the immunogenicity of a recombinant M. tuberculosis Ag85C-MPT51-HspX fusion protein (CMX) in mice and individuals with active tuberculosis. BALB/c mice were immunized with the CMX protein liposome-encapsulated with CpG DNA or with CpGDNA liposome-encapsulated, liposome or saline as negative controls. The immunization produced high levels of anti-CMX -specific IgG1 and IgG2a antibodies and induced an increase in the relative and absolute numbers of specific TCD4 IFN-c+ and TNF-a+ cells in the spleen. Sera from a cohort of individuals with active tuberculosis contained higher levels of IgG and IgM that recognized CMX when compared to healthy individuals. In conclusion, this protein was shown to be immunogenic both in mice and humans.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: SOUSA, Eduardo Martins de; COSTA, Adeliane Castro da; TRENTINI, Monalisa Martins; ARAÚJO FILHO, João Alves de; KIPNIS, André; JUNQUEIRA-KIPINIS, Ana Paula. Immunogenicity of a fusion protein containing immunodominant epitopes of Ag85C, MPT51, and HspX from Mycobacterium tuberculosis in mice and active TB infection. Plos One, San Francisco, v. 7, n.10, p. e47781, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pone.0047781
Identificador do documento: 10.1371/journal.pone.0047781
URI: http://repositorio.bc.ufg.br/handle/ri/16567
Data de publicação: 25-Out-2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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