Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations
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Data
2015-12
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Resumo
Handling equine semen during the refrigeration process reduces sperm viability, and consequently
causes membrane lipid peroxidation, among other challenges. The present study aimed to evaluate the
in vitro effects of glutathione (control, 1. 0, 1. 5, and 2. 5 mM) on equine semen in a cooled protocol of
16ºC for 36 hours. The following variables were evaluated after 0, 12, 24, and 36 hours refrigeration:
total sperm motility, vigor, viability, and plasma and acrosomal membrane integrity. Motility was higher
with 2. 5mM of glutathione (57. 8 ± 7. 3) after 12 hours of refrigeration compared to the control (53. 2
± 8. 3) (P < 0. 05). After 36 hours of refrigeration, motility was higher with 1. 5 mM (43. 4 ± 12. 7) and
2. 5mM glutathione (45. 5 ± 6. 2), than it was with 1mM glutathione (38. 2 ± 9) and the control (35. 5
± 18. 4) (P < 0. 05), respectively. The strength was highest with 1. 5mM glutathione (3. 7 ± 0. 3) after
36 hours compared to the control (3. 2 ± 1. 1), (P < 0. 05). Viability differed between control and 1mM
treatments (79. 5 ± 1. 8) only after 24 hours (75. 5 ± 9. 7) (P < 0. 05). Throughout the investigation, no
significant differences were noted in plasma and acrosomal membrane integrity (P > 0. 05). The 1. 5 and
2. 5mM glutathione levels were more efficient in protecting sperm cells and yielded higher total motility
values after 36 hours of refrigeration.
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Antioxidant, Sperm, Stallion, Cooled semen, Antioxidante, Espermatozoide, Garanhão, Sêmen refrigerado
Citação
OLIVEIRA, Rodrigo Arruda de; VIU, Marco Antônio de Oliveira; GAMBARINI, Maria Lúcia. Cooling of equine semen at 16°C for 36 hours with addition of different glutathione concentrations. Semina: ciências agrárias, Londrina, v. 36, n. 6, p. 3699-3704, Nov./Dec. 2015.