Avaliação do potencial antioxidante e atividade vasorrelaxante de Cinnamomum sp.
Nenhuma Miniatura disponível
Data
2023-05-16
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
The great interest in the study of antioxidants is mainly the prevention of
physiological damage caused by oxidative processes. Cinnamon (Cinnamomum
sp.), is a spice used worldwide in cooking and has gained prominence for its
pharmacological properties, such as anti-inflamatory, antibacterial, antifungal,
cardiovascular, among others. Given the importance of determining the antioxidant
capacity in relation to therapeutic methods, this study aims to evaluate the
antioxidant profile of commercial cinnamon samples by spectrophotometric,
electrochemical and vasorelaxant methods. The spectrophotometric methods
performed were 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis (3-
ethylbenzothiazoline-6-sulfonic acid) (ABTS) and Folin-Ciocalteu. For the
electrochemical experiments, a three-electrode system was used, consisting of
carbon paste electrode, platinum wire, and Ag/AgCl/KClsat, representing the
working, auxiliary, and reference electrodes, respectively. The electroanalytical
methods used were differential pulse, square wave, and cyclic voltammetries.
Vascular reactivity was evaluated in aortic artery rings from male Wistar rats, using
arteries with intact vascular endothelium and without endothelium. The endothelial
nitric oxide synthase (eNOS) pathway and the inhibitor N-nitro-L-arginine-methylester
(L-NAME) were used in this analysis. The correlation between the
electrochemical approach and total phenols by the ABTS, DPPH, and Folin-
Ciocalceu methods were 0.63, 0.70, and 0.73, respectively, with 1 being an ideal
correlation. The correlation between spectrophotometric methods was 0.83. For the
electrochemical results, a similar profile was observed, with anodic peak a.c Epa1=
0.1 V, indicative of polyphenolic compounds with high antioxidant power. In
addition, a biosensor on a carbon paste electrode was applied to the selected
extracts using laccase enzyme, obtained by the fungus Marasmiellus sp. and a
signal improvement of up to 4 times in the antioxidant profile was observed when
compared to the electrode without modification. The samples were analyzed by
mass spectrometer, and the main chemical markers found were coumarin,
cinnamaldehyde, and eugenol. Pharmacological assays showed that these samples
also promoted a significant vasorelaxant effect associated to the antioxidant
potential. Thus, cinnamon showed a high antioxidant capacity, in agreement with
the results obtained in other studies, emphasizing its importance as a functional
food.
Descrição
Palavras-chave
Citação
GONÇALVES, E. K. Avaliação do potencial antioxidante e atividade vasorrelaxante de Cinnamomum sp. 2023. 81 f. Tese (Doutorado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2023.