Qualidade do sêmen equino criopreservado com L-acetil-cisteina
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Data
2014-08-27
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Universidade Federal de Goiás
Resumo
This study was conducted to evaluate the effect of adding L-acetyl-cysteine (CIS) as
anti oxidant agent to extender used for sperm cryopreservation of equine Mangalarga
Marchador. Three semen samples from seven healthy stallions were obtained by
artificial vagina, diluted in commercial extender and distributed into two treatments:
control without addition and CIS, containing the antioxidant concentration of 2.5 mM.
After the stabilization period, the samples were filled into 0.5 mL straws and
subjected to cryopreservation protocol manually. Sixty days after the samples were
thawed and evaluated in vitro by means of computerized analysis of sperm kinetics
(CASA) and the evaluation of the integrity of plasma and acrosomal membranes by
fluorescent probes. Regarding the kinetic variables related to sperm was found that
in samples supplemented with CIS percentage of total and progressive motility (TM)
(MP) was higher (P <0.01) compared to control samples (23.95±3 , 33 X 11 .38±2.35
and 4.66 ± 0.84 X 2.04 ± 0.36, respectively), but variable for straightness (STR) and
linearity (LIN) the percentages were highe r (p ≤ 0.01) for the control samples (86.95 ±
1.02 X 82.28 ± 1.02 and 49.85 ± 1.32 X 45.19 ± 1.17, respectively). As those related
to the integrity of the membranes of the sperm variables was superior (P <0.05) in
the number of sperm with intact acrosome reaction (ICRA) in the samples compared
to those CIS Control (4.7 ± 1.6 x 0.8 ± 0.2, respectively). The results suggest that the
addition of acetyl-L-cysteine concentration of 2.5 mM in the diluent medium before
cryopreservation can improve both the motility as the integrity of the sperm plasma
membrane equine Mangalarga Marcher. More studies should be done to confirm this
effect in vivo.
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FRANCISCO JÚNIOR, Arthur. Qualidade do sêmen equino criopreservado com L-acetil-cisteina. 2014. 34 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2014.