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- Campus Samambaia, Campus Colemar Natal e Silva, Campus Aparecida de Goiânia.
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Proteômica: uma ferramenta analítica
(2019) Guissoni, Ana Carla Peixoto; Cardoso, Divina das Dôres de Paula
Proteomics is an analytical technique that allows the determination of proteins in a
biological sample. Proteomic analysis is one of the most used techniques to study
proteins differentially expressed in a cell when infected by microorganisms. In this way,
the proteomic analysis can provide important information on the pathogen-cell
relationship, pathogenesis of the microorganism, as well as contribute to the
identification of target molecules that may be useful both for the development of
vaccines as well as for markers, diagnostics and therapeutics. This manuscript
presents a summary of different methodologies with a proteomic approach which have
been used for the study of differentially expressed proteins from cells infected or
uninfected by microorganisms.
Circulation profile of respiratory viruses in symptomatic and asymptomatic children from Midwest Brazil
(2020) Castro, Ítalo de Araújo; Costa, Lusmaia Damaceno Camargo; Oliveira, Anniely Carvalho Rebouças; Souza, Menira Borges de Lima Dias e; Cardoso, Divina das Dôres de Paula; Camargos, Paulo Augusto Moreira; Costa, Paulo Sérgio Sucasas; Fiaccadori, Fabíola Souza
Acute respiratory infection (ARI) is a major cause of morbidity and mortality worldwide. Most of these infections are caused by
viruses. Infections pose as important triggers of acute episodes of chronic respiratory diseases (CRD). This study sought to
evaluate the frequency and circulation profile of respiratory viruses among ARI symptomatic patients and completely asymp tomatic children in Midwest Brazil. The study enrolled symptomatic children with and without ARI symptoms. During 1 year,
225 nasal respiratory samples were obtained from patients aged 4–14 years old. The samples were screened by multiplex nested PCR for 16 common respiratory viruses. From 225 samples, 42 had at least one virus detected. Samples from four different
patients had multiple viruses detected. The viral detection rate in symptomatic (20.1%) and asymptomatic patients (14.8%)
showed no significant difference. The most frequent viruses detected were rhinovirus (28.6%), FLUA (11.9%), adenovirus
(11.9%), human bocavirus (HBoV) (11.9%), and respiratory syncytial virus (RSV) antigenic group A (9.5%). Monthly detection
rate was higher during the rainy season. RSVs were detected during the months with higher rainfall indexes and higher air
humidity, while FLU and HBoV were detected during the winter months. The obtained results reinforce the importance of viral
pathogens in pediatric population, emphasizing similar viral occurrence in symptomatic and asymptomatic children.
Adenovirus infection among allogeneic stem cell transplant recipients
(2016) Santos, Hugo César Pereira; Almeida, Tâmera Nunes Vieira; Fiaccadori, Fabíola Souza; Cardoso, Divina das Dôres de Paula; Arantes, Adriano de Moraes; Silva, Hugo Delleon da; Nagib, Patricia Resende Alo; Souza, Menira Borges de Lima Dias e
The human adenovirus (HAdV) infection can cause severe disease in immunocompromised patients, such as those undergoing allogeneic hematopoietic stem cell transplant (ASCT). The main objective of this study was to prospectively monitor ASCT recipients for HAdV occurrence in a reference center in Brazil, and also to correlate viral positivity, viral load, molecular variant, clinical symptoms, and patients’ prognosis. From October/2012 to October/2014, blood and feces of 21 ASCT recipients were screened for HAdV by Nested-PCR. Viral loads were determined by real-time PCR. In total, 57% of the patients had at least one positive sample (serum or feces) for HAdV. Patients presented significantly higher viral load in feces when compared to serum. Positive samples were characterized as HAdVs of species HAdV-C, -D, and -F. The main clinical symptom presented by infected patients was diarrhea, and Graft-versus-host disease (GVHD) was the main intercurrence. An association was observed between HAdV-positivity and diarrhea and also between HAdV-positivity and GVHD. Results from this study may contribute to a better understanding of the HAdV infection pattern in patients submitted to ASCT. Data therein highlight the importance of including HAdV testing during all routine laboratory exams performed on ASCT patients.
Single-dose varicella vaccine effectiveness in Brazil: a case-control study
(2018) Andrade, Ana Lúcia Sampaio Sgambatti de; Vieira, Maria Aparecida da Silva; Minamisava, Ruth; Toscano, Cristiana Maria; Souza, Menira Borges de Lima Dias e; Fiaccadori, Fabíola Souza; Figueiredo, Cristina Adelaide; Curti, Suely Pires; Nerger, Maria Lígia Bacciotte Ramos; Bierrenbach, Ana Luiza de Souza
Background: Varicella vaccine was introduced into the Brazilian Immunization Program in October 2013,
as a single-dose schedule administered at 15 months of age. Its effectiveness had not yet been assessed in
the country.
Methods: A matched case-control study was carried out in São Paulo and Goiânia (Southeast and Midwest
regions, respectively), Brazil. Suspected cases, were identified through a prospective surveillance established in the study sites. All cases had specimens from skin lesion collected for molecular laboratory testing. Cases were confirmed by either clinical or PCR of skin lesions and classified as mild, moderate, and
severe disease.
Methods: Two neighborhood controls were selected for each case. Cases and controls were aged 15–32
months and interviewed at home. Evidence of prior vaccination was obtained from vaccination cards.
Univariate and multivariate logistic regression models were used, and odds ratio and its respective
95% confidence intervals were estimated. Vaccine effectiveness was estimated by comparing de odds
of having received varicella vaccine among cases and controls.
Results: A total of 168 cases and 301 controls were enrolled. Moderate and severe illness, was found in
33.3% and 9.9% of the cases. Effectiveness of a single dose varicella vaccine was 86% (95%CI 72–92%)
against disease of any severity and 93% (95%CI 82–97%) against moderate and severe disease. Out of
168 cases, 81.8% had positive PCR results for wild-type strains, and 22.0% were breakthrough varicella
cases. Breakthrough cases were milder compared to non-breakthrough cases (p < .001).
Conclusions: Effectiveness of single dose varicella vaccine in Brazil is comparable to that in other countries where breakthrough varicella cases have also been found to occur. The goal of the varicella vaccination program, along with disease burden and affordability should be taken into consideration when
considering the adoption of a second dose of varicella vaccine into national immunization programs.
Identification of Human Bocavirus type 4 in a child asymptomatic for respiratory tract infection and acute gastroenteritis – Goiânia, Goiás, Brazil
(2017) Sousa, Teresinha Teixeira de; Almeida, Tâmera Nunes Vieira; Fiaccadori, Fabíola Souza; Souza, Menira Borges de Lima Dias e; Badr, Kareem Rady Abdelhaleem; Cardoso, Divina das Dôres de Paula
Human Bocavirus (HBoV) has been identified from feces and respiratory samples from cases
of both acute gastroenteritis and respiratory illness as well as in asymptomatic individuals.
The aim of this study was to detect and characterize HBoV from fecal samples collected
from hospitalized children aged less than five years old with no symptoms of respiratory
tract infection (RTI) or acute gastroenteritis (AGE). The study involved 119 children and one
fecal sample was collected from each participant between 2014 and 2015. HBoV was detected
using Nested-PCR, and the viral type identified by genomic sequencing. HBoV-4 was iden tified from one sample obtained from a hospitalized child with soft tissue tumor of the
submandibular region. This is the first report of HBoV-4 identification in Brazil, but we con sider that this type may be circulating in the country similar to the other types and new
investigations are necessary.