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Item type: Item , Suspected glyphosate poisoning in a dog(2025) Souza, Marcelino Benvindo de; Silva, Rayanne Henrique Santana da; Almeida, Sara Sueli Ferreira de; Leopoldino, Cilho Gabriel Rodrigues; Leopoldino, Ana Gabriela Rodrigues; Passos, Pedro Henrique Miranda; Paula, Luiza Gabriella Ferreira de; Silva, Daniela de Melo e; Martins, Danieli Brolo; Botelho, Ana Flávia MachadoGlyphosate is a widely used pesticide that has been associated with poisoning in both humans and animals. Its toxicity remains a topic of controversy, particularly regarding its carcinogenic potential. This study aimed to investigate a suspected case of glyphosate poisoning in a dog, providing a detailed account of the case history, clinical evolution, laboratory findings, and genotoxic assessment. A seven-month-old dog was referred to the Veterinary Hospital, presenting with mental confusion and ataxia. The pet guardian reported altered consciousness and incoordination twelve days after Roundup® was applied to the farm’s grass. A complete blood count revealed normocytic normochromic anemia, a packed cell volume of 20%, thrombocytopenia (50,000/µL), and severe leukocytosis (447,200/µL), with 95% of the white blood cells consisting of blasts of unidentified origin and 5% segmented neutrophils. Myelogram analysis showed a moderately cellular bone marrow sample with a predominance of primitive cells of undetermined origin (>95%), suggesting a diagnosis of leukemia. To further investigate the potential link between leukemia and glyphosate exposure, whole blood was collected in a heparinized tube for a comet assay. The test indicated moderate DNA damage. Chemotherapy was recommended; however, treatment was not pursued for personal reasons, and the patient died five days after the consultation. A necropsy was not authorized. This report contributes to the understanding of glyphosate poisoning and its potential health consequences, particularly its association with hematologic malignancies in canines.Item type: Item , Experimental assessment of beauvericin toxicity in tadpoles of Dendropsophus minutus (Anura: Hylidae)(2025) Vicente, Monica Chacon de; Paula, Luiza Gabriella Ferreira de; Freitas, Renata Maria Pereira de; Almeida, Adieli dos Anjos; Lima, Diego da Silva; Gabriel, Rebeca dos Santos; Nogueira, Nathany Geraldino; Batista, Nádya Raquel dos Santos; Barros, Alexandre Có Mangoni; Souza, Marcelino Benvindo de; Bastos, Rogerio Pereira; Brito, Pedro Vale de Azevedo; Silva, Daniela de Melo e; Botelho, Ana Flávia MachadoBeauvericin (BEA) is an emerging mycotoxin frequently detected in food and feed, raising concerns about its toxicological risks for One Health. This study evaluated the acute toxicity of BEA in tadpoles of Dendropsophus minutus, a bioindicator species of environmental toxicity, after a 96-h exposure. Tadpoles were exposed to three concentrations of BEA, with vehicle (DMSO), positive (cyclophosphamide), and negative (water) controls. Outcomes included survival, genotoxicity (comet assay), mutagenicity (micronucleus test), leukocyte profiles, redox status markers, and liver histopathology. Exposure to BEA did not induce genotoxicity; however, it resulted in mutagenic effects, with an increase in reniform nuclei and a decrease in anucleated erythrocytes, and hepatotoxicity characterized by sinusoidal congestion, reduced hepatocyte cytoplasmic volume, and increased connective tissue. Elevated levels of nitric oxide and carbonyl proteins were observed, indicating oxidative stress, while antioxidant enzymes and acetylcholinesterase activity remained unchanged. These findings demonstrate that BEA induces mutagenic, cytotoxic, and hepatotoxic effects in amphibians, highlighting its potential ecological impact and relevance for One Health risk assessment.Item type: Item , Influence of temperature and storage time on the stability of biochemical parameters in broilers(2025) Cifuentes Ruiz, Katalina; Qualhato, Andriele Ferreira; Albuquerque, Luis Fernando Duarte; Trentin, Thays de Campos; Santos, Ronan Rodrigues dos; Barragán Ruiz, Carmen Elena; Marín Oviedo, Gonzalo; Café, Marcos Barcellos; Botelho, Ana Flávia Machado; Martins, Danieli BroloPoultry farming is a strategic sector of Brazilian agribusiness, requiring high health standards to ensure productivity and competitiveness. Biochemical tests are essential for monitoring poultry health, but pre-analytical variables can compromise their results. This study evaluated the influence of storage time and temperature on the stability of 14 serum analytes in COBB 500 broilers, both males and females, aged 35 and 40 days. Samples were analyzed under different conditions: room temperature (27 ± 2°C), refrigeration (6 ± 2°C), freezing (−20 ± 5°C), and ultra-freezing (−80 ± 5°C), over periods of up to 365 days. Analyte stability varied according to the analyte and storage condition. Lactate was the only parameter that remained stable under all tested condition. It is concluded that the preservation of biochemical analytes depends directly on storage conditions, which must be carefully considered to ensure the reliability of results in healthy broilers.Item type: Item , Correlation between the ultrasonographic measurement of intestinal thickening and laboratory parameters in asymptomatic dogs(2025) Oliveira, Iago Martins; Almeida, Sara Sueli Ferreira de; Sprícigo, Carolle Vieira Muterlle; Silva, Wanessa Patrícia Rodrigues da; Silva, Marco Augusto Machado; Botelho, Ana Flávia Machado; Martins, Danieli Brolo; Borges, Naida CristinaBackground: Intestinal ultrasound is a valuable diagnostic tool for gastrointestinal diseases in dogs. Despite its usefulness, the clinical significance of intestinal thickening in asymptomatic animals remains unclear. Aim: This study aimed to correlate the thickness of the intestinal layers with hematological and biochemical parameters in asymptomatic dogs. Methods: Thirty healthy dogs were assessed and divided into two groups: G1 (n = 15), with no ultrasound alterations, and G2 (n = 15), with different intestinal thicknesses. Pearson’s correlation test was used to assess possible associations between laboratory data and intestinal thickening. Results: The results showed no statistically significant correlation between intestinal thickness and keratometric parameters, leukometry, proteinogram, and serum biochemistry (r ranging from -0.004 to 0.29 in hematology and from 0.008 to 0.2 in biochemistry, with p > 0.05). Conclusion: Therefore, identifying early markers for the diagnosis of enteropathies in asymptomatic dogs was not possible. The lack of associations limits the clinical usefulness of intestinal ultrasound as a predictor of laboratory alterations or as a tool for diagnosing enteropathies in the early stages. Expanding the sample, using additional biomarkers, conducting prospective studies, and correlating ultrasound findings with intestinal histology are recommended. These steps could help explore new clinical and laboratory markers to better understand the clinical significance of gastrointestinal tract thickening in dogs.Item type: Item , Evaluation of Scalibor® deltamethrin collar chronic exposure in dogs(2025) Rodrigues, Larissa T. P.; Qualhato, Andriele Ferreira; Sprícigo, Carolle Vieira Muterlle; Arantes, Kelly Carolina Assis; Silva, Rayanne Henrique Santana da; Barros, Alexandre Có Mangoni; Vicente, Monica Chacon de; Souza, Murilo R.; Paula, Luiza Gabriella Ferreira de; Campos, Danilo Roman; Botelho, Ana Flávia MachadoDeltamethrin is a pesticide used worldwide with several applications in the control of nuisance arthropods in homes, agriculture, against ectoparasites in dogs and livestock. Recent studies indicate that deltamethrin can induce cardiotoxicity. Therefore, this study aimed to evaluate the toxicity of deltamethrin in dogs through exposure to commercial collars over 8 months. Twenty healthy dogs were selected, 9 males and 11 females, weighing between 10 and 15 kg and aged between 1 and 8 years, with no history of intentional exposure to pyrethroids. Clinical, cardiological, and laboratory evaluations were performed monthly. The results demonstrate that exposure to deltamethrin 4 % via the dermal route, through a commercial collar, was safe for 8 months, with preserved cardiac electrocontractile function, assessed by electrocardiogram and echocardiogram. In addition, tracking of plasma biochemical markers did not indicate signs of cardiac, renal, or hepatic injury. Although deltamethrin 4 % collars demonstrated an acceptable safety profile regarding cardiovascular and laboratory evaluations, the occurrence of dermatological reactions in 25 % of the animals, which is a significant proportion, highlights the need for careful monitoring of dermatological reactions. Future investigations with different concentrations and longer periods are recommended to achieve a more comprehensive understanding of the long-term effects of deltamethrin, improving its safe application in veterinary medicine and promoting animal and human health.