Navegando por Autor "Costa, Dayane de Melo"
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Item Análise bacteriológica de staphylococcus spp. isolados de placas ortopédicas implantáveis, antes do processamento pelo serviço de saúde(Universidade Federal de Goiás, 2019-12-12) Carvalho, Amanda Soares de; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Vieira, José Daniel GonçalvesHealthcare Related Infections (IRAS) represent a serious public health problem and are among the leading causes of morbidity and mortality in the world. Among the types of HAI are Surgical Site Infections (SSIs) that may be due, for example, to the use of contaminated health products. In orthopedic surgeries, the occurrence of SSI is a serious complication for patients and may be associated with the use of contaminated implantable orthopedic plates. As these are critical health products that come into contact with tissues that do not have their own microbiota, they must be submitted to the sterilization process prior to use. However, failures in the processing steps, especially cleaning associated with biofilm formation in these devices, may prevent the implant from being properly sterilized. This study aimed to characterize the contamination profile of implantable orthopedic plaques by Staphylococcus spp., Before processing by the health service, and was carried out at a public teaching hospital in Goiânia, Goiás, from May to December 2018. Five Orthopedic surgical implant boxes called “Small Fragments”, provided by a consignment / lending system company, were selected 15 smaller implantable orthopedic plates collected using aseptic technique. They were submitted to bacteriological analysis for isolation and phenotypic identification of Staphylococcus spp., As well as evaluation of antimicrobial susceptibility profile. Among the evaluated implantable orthopedic plates, four (26.7%) were contaminated with Staphylococcus spp., And a total of four isolates were recovered. The most isolated species was Staphylococcus hycus (50.0%). Staphylococcus epidermidis (25.0%) and coagulase-negative Staphylococcus (25.0%) were also isolated. The microorganisms were sensitive to the evaluated antimicrobials, except Staphylococcus hyicus, which was resistant to cefoxitin, predicting methicillin / oxacillin resistance. These data point to the importance of properly performing the processing steps of these implants, considering the capacity of isolated microorganisms to form biofilm, especially when the implant presents non-conformities such as grooves, dirt, oxidation and other factors. The formation of biofilms in these devices may lead to the occurrence of chronic, severe and difficult to treat infections.Item Colonização de trabalhadores de áreas de apoio hospitalar por staphylococcus sp.: aspectos epidemiológicos e microbiológicos(Marcelo Medeiros, 2016-03-31) Vilefort, Larissa Oliveira Rocha; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Lima, Lima; Peleja, Edgar Berquó; Prado, Marinésia Aparecida doItem Contaminação de equipamentos e superfícies de unidades de terapia intensiva de uma maternidade pública por staphylococcus coagulase negativa(Ruy de Souza Lino Junior, 2013) Moraes, Carolina Leão de; Ribeiro, Nádia Ferreira Gonçalves; Costa, Dayane de Melo; Furlan, Vinícius Gontijo; Palos, Marinésia Aparecida Prado; Vasconcelos, Lara Stefânia Netto de Oliveira LeãoIntensive care units are considered critical areas of a hospital, due to patient’s high risk for infections. The setting of these units constitutes a potential reservoir of pathogenic microorganisms, which may be conveyed to susceptible hosts such as newborns and children. The aim of this study was to detect the presence of coagulase-negative Staphylococcus on surfaces and equipment in the intensive care units of a public maternity ward in Goiânia, Goiás. The research was conducted in three units, two pediatric and one neonatal. Sample collection occurred from September/2011 to July/2012. We assessed 93 materials, 48 items of equipment and 45 surfaces. The procedure for collection of samples was performed using a swab, which was inoculated into specific culture medium. The bacterial isolates were identified by microscopic analysis, macroscopic and biochemical tests. Among the materials tested, 62 (66.7%) were contaminated with coagulase-negative Staphylococcus, while 32 items of equipment (66.7%) and 30 surfaces (66.7%) were also contaminated. The incubators (90.9%) and countertops (88.9%) were the most frequently contaminated materials. A total of 65 bacteria were isolated. Some materials (n=3) were contaminated with two concomitant bacterial strains, but phenotypically different. In this study, the equipment and surfaces investigated were identified as a focus of contact for coagulase-negative Staphylococcus, presenting a risk for the transmission of microorganisms to newborns and hospitalized children. The adoption of procedures for more effective decontamination are necessary to control the spread of the bacteria in the hospital environment.Item Enterobacteriaceae isolates from the oral cavity of workers in a brazilian oncology hospital(2015-04) Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; Oliveira, Ana Claúdia Alves de; Gonçalves, Nádia Ferreira; Vieira, José Daniel Gonçalves; Prado, Marinésia Aparecida doThe evaluation of workers as potential reservoirs and disseminators of pathogenic bacteria has been described as a strategy for the prevention and control of healthcare-associated infections (HAIs). The aim of this study was to evaluate the presence of Enterobacteriaceae in the oral cavity of workers at an oncology hospital in the Midwest region of Brazil, as well as to characterize the phenotypic profile of the isolates. Saliva samples of 294 workers from the hospital’s healthcare and support teams were collected. Microbiological procedures were performed according to standard techniques. Among the participants, 55 (18.7%) were colonized by Enterobacteriaceae in the oral cavity. A total of 64 bacteria were isolated, including potentially pathogenic species. The most prevalent species was Enterobacter gergoviae (17.2%). The highest rates of resistance were observed for β-lactams, and 48.4% of the isolates were considered multiresistant. Regarding the enterobacteria isolated, the production of ESBL and KPC was negative. Nevertheless, among the 43 isolates of the CESP group, 51.2% were considered AmpC β-lactamase producers by induction, and 48.8% were hyper-producing mutants. The significant prevalence of carriers of Enterobacteriaceae and the phenotypic profile of the isolates represents a concern, especially due to the multiresistance and production of AmpC β-lactamases.Item Infecções associadas ao cuidado em saúde em um hospital oncológico brasileiro: análise de cinco anos(2012-01) Santos, Silvana de Lima Vieira dos; Sousa, Thais Kato de; Costa, Dayane de Melo; Lopes, Lillian Kelly de Oliveira; Peleja, Edgar Berquó; Melo, Dulcelene de Sousa; Prado, Marinésia Aparecida do; Pereira, Milca SeverinoEstudio epidemiológico retrospectivo realizado en 2009 a fin de analizar las tasas de infección asociada a los cuidados en la salud, en pacientes oncológicos, por topografía, letalidad y mortalidad de 2004 a 2008. Los datos se recogieron de registros archivados en el Servicio de Control de Infección Hospitalaria (SCIH) de la institución. Los aspectos éticos fueron observados. Referente a la tasa de infección asociada a los cuidados en Enfermería Global Nº 25 Enero 2012 Página 19 salud se observó que ocurrió el 8,24% (5.821) de episodios en el período. Estas tasas presentaron variación del 6,51% (1,017) en 2004 al 10,82% (1.790) en 2007 y desvío estándar = (± 1,91). Referente al número de pacientes con infección se encontró el 5,75% (4.064) variando del 4,89% (765) en 2004 al 7,47% (1237) en 2007. Las topografías más acometidas fueron las de sitio quirúrgico con el 26,11%, corriente sanguínea con el 24,11% y el tracto respiratorio con el 18,50%. La tasa de letalidad y mortalidad asociadas a la infección fueron el 23,86% y el 1,37% de óbitos respectivamente. Se considera necesaria la evaluación de los múltiples factores involucrados en ese proceso para que la institución desarrolle estrategias de prevención y control de las infecciones y pueda intervenir en tiempo hábil.Item Methicillin-resistant Staphylococcus sp. colonizing health care workers of a cancer hospital(2014-09) Costa, Dayane de Melo; Kipnis, André; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Vilefort, Larissa Oliveira Rocha; Teles, Sheila Araujo; André, Maria Cláudia Dantas Porfirio Borges; Tipple, Anaclara Ferreira Veiga; Lima, Ana Beatriz Mori; Ribeiro, Nádia Ferreira Gonçalves; Pereira, Mayara Regina; Prado, Marinésia Aparecida doThe aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and sa- liva sampling were performed with 149 health care workers. Antimicrobial resistance was deter- mined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were per- formed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were de- termined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and administration of antimicrobial (p = 0.04) were risk factors identified. All methicillin-resistant Staphylococcus were S. epidermidis, 94.4% of them had mecA gene. Closely related and indistinguishable methicillin- resistant S. epidermidis were detected. These results highlight that HCWs which have contact with patient at high risk for developing infections were identified as colonized by MRSE in the oral cavity, reinforcing this cavity as a reservoir of these bacteria and the risk to themselves and patients safety, because these microorganisms may be spread by coughing and talking.Item Perfil dos trabalhadores de um hospital oncológico colonizados na cavidade bucal por enterobacteriaceae(Ruy de Souza Lino Junior, 2014-08-13) Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Lima, Ana Beatriz Mori; Costa, Dayane de Melo; Rocha-Vilefort, Larissa Oliveira; Oliveira, Ana Cláudia Alves de; Vieira, José Daniel Gonçalves; Prado, Marinésia Aparecida do,The aim of this study was to characterize the profile of workers of the oncology hospital colonized by Enterobacteriaceae in the oral cavity. Along with bacterial colonization, socio-demographic, professional, disease/ infection and behavioral variables were investigated. The collection of saliva and microbiological analyses were performed using standard techniques. Data collection was conducted through a questionnaire. Among the 55 professionals colonized by Enterobacteriaceae, 56.4% (31/55) also harbored Staphylococcus and/or Pseudomonas in the oral cavity. The most commonly infected professionals were nursing technicians. Episodes of disease/infection were reported among carriers. The data revealed a worrying situation in the context of healthcare. It is hoped that these results will contribute to the prevention and control of this infection, as knowledge of carrier status reduces the risk of microorganism transmission.Item Protocolo para processamento de endoscópios flexíveis: qualificando a prática clínica(Cegraf UFG, 2022) Azevedo, Adriana da Silva; Tipple, Anaclara Ferreira Veiga; Oliveira, Ellen Synthia Fernandes de; Primo, Mariusa Gomes Borges; Guadagnin, Simone Vieira Toledo; Costa, Dayane de Melo; Melo, Dulcelene de Sousa; Luciano, Cristiana da Costa; Neves, Heliny Carneiro Cunha; Azevedo (org.), Adriana da Silva; Tipple (org.), Anaclara Ferreira Veiga; Oliveira (org.), Ellen Synthia Fernandes de; Rosso (org.), Claci Fátima WeirichItem Pseudomonas spp. isolated from the oral cavity of healthcare workers from an oncology hospital in midwestern Brazil(2015-12) Lima, Ana Beatriz Mori; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; Costa, Dayane de Melo; Vilefort, Larissa Oliveira Rocha; André, Maria Cláudia Dantas Porfirio Borges; Barbosa, Maria Alves; Prado, Marinésia Aparecida doThis cross-sectional study, performed in an oncology hospital in Goiania, aimed to characterize the prevalence of oral colonization and antimicrobial susceptibility of Pseudomonas spp. isolated from the saliva of healthcare workers. Microorganisms were subjected to biochemical tests, susceptibility profile, and phenotypic detection. Of 76 participants colonized with Gram negative bacilli, 12 (15.8%) harbored Pseudomonas spp. Of all isolates, P. aeruginosa (75.0%), P. stutzeri (16.7%), and P. fluorescens (8.3%), were resistant to cefoxitin, and therefore likely to be AmpC producers. The results are clinically relevant and emphasize the importance of surveillance to minimize bacterial dissemination and multiresistance.