Novo meio liquido autoclavável isento de sangue, para isolamento primário de leishmanias de lesões tegumentares: i - sua potencialidade no estudo da biologia dos tripanosomatideos
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Data
1984-04
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Editor
Ruy de Souza Lino Junior
Resumo
A new culture médium, first called
BHYM1 (Brain heart-yeast-meat infusion)
then simply called Rq, consistíng basically
of brain extract, heart extract, meat
extract, yeast extract glucose, sodium
chloride and water is an inexpensive monophasic médium commercially available,
easy to prepare and to sterilize. It was
used first as the liquid phase of the diphasic
media NNN or Ducrey and then as an
exclusive culture médium for leishmania.
It ailous the isolation of leishmania
from lesions and the maintenance of isolated
strains. It is stable for 12 months
at room temperature in the liquid state.
Its optimal pH is 7.4 to 7.6.
When enriched with fetal bovine
se rum in the proportion of 5, 10, 20 and
or 30%, it increases growth of strains
of leishmania and allows cultivation of
Trypanosoma sp; when used at higher
temperaturas it leads to the appearance
of amastigote forms of Trypanosomes.
When assocíated with ducle serum
and/or in the presence of tetracycline hydrochloride,
metacyclogenesis of Trypanosoma
cruzi is stimulated.
When it is associated with chicken
blood (plasma and/or serum) it stimulates
transformation of promastigotes of leishmania
sp to amastigotes at proper temperature
(350C).
Luriched with charcoal from fetal
or adult bovine blood, and from ducle
blood if leads to profuse growth of
Trypanosome and is very good for isolation
maintename and growth of Trypanosoma
cruzi.
This médium can also be used as a
diphasic médium incorporated to peptone
or agar and having as a liquid médium the
médium itself. bovine serum, fetal bovine
serum or ducle serum, médium 199,
médium of Grace or liquid supplement of
Hanks, according to the purpose of cultivation.
The association of extract from
Phlebotomidae pr troatp, odae tp tje
media increased the growth of leishmania.
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BARBOSA, William; CZEREWUTA, Ana Cândido; OLIVEIRA, Osvaldira Seabra; OLIVEIRA, Raquel Lopes de. Novo meio liquido autoclavável isento de sangue, para isolamento primário de leishmanias de lesões tegumentares: i - sua potencialidade no estudo da biologia dos tripanosomatideos. Revista de Patologia Tropical, Goiânia, v. 13, n.1, p. 9-24, jan./abr. 1984. Disponível em: < https://www.revistas.ufg.br/iptsp/article/view/21269/12484>.