Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates
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2011-12
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Introduction: Candida albicans is responsible for superficial or systemic infections known as
candidiasis, which may be found in infected tissue as unicellular budding yeasts, hyphae, or
pseudohyphae. In this study, the effects of both fluconazole and itraconazole antifungal agents
on the hyphal formation and genotypic characterization of C. albicans isolates classified as either
susceptible or resistant were investigated. Methods: The hyphal production of five C. albicans
isolates under the action of antifungal agents was investigated by culturing yeast on growth
medium and on hyphal induction medium. The genotypic characterization was carried out
for 13 isolates of C. albicans using the random amplified polymorphic DNA-polymerase chain
reaction (RAPD-PCR) method. Results: The dimorphism analysis showed that the hyphal
formation was higher in resistant than in the susceptible isolates to both azoles. The RAPD-PCR
method identified the formation of two different groups. In group A, four resistant and two
susceptible isolates were clustered, and in group B, one resistant and six susceptible isolates
were clustered. Conclusions: Considering that hyphal formation was higher in resistant isolates
in the presence of azole drugs, we confirmed that the hyphal production is closely related to
susceptibility to azoles. These drugs may affect the morphogenesis of C. albicans depending on
their susceptibility to these drugs. In relation to RAPD-PCR, most resistant isolates classified
in group A and susceptible isolates in group B demonstrated that this method presented a
similar standard between the two groups, suggesting that by this technique, a strong correlation
between genotypes and fluconazole-resistant samples may be found.
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Candida albicans, RAPD-PCR, Azole antifungals, Hyphal formation, In vitro susceptibility, Candida albicans, Antifúngicos azólicos, Suscetibilidade in vitro, RAPD-PCR, Formação hifal
Citação
COSTA, Carolina Rodrigues; SOUZA, Lúcia Kioko Hasimoto e ; ATAÍDES, Fábio Silvestre; FERRI, Pedro Henrique; COSTA, Maysa Paula da; FERNANDES, Orionalda de Fátima Lisboa; SILVA, Maria do Rosário Rodrigues. Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 44, n. 6, p. 740-744, Nov./Dec. 2011.