Biofilm forming capability and antifungal susceptibility profile of candida spp. from blood
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Data
2018-03
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Editor
Ruy de Souza Lino Junior
Resumo
Although Candida albicans remains the most frequent Candida species; however other
species have emerged as important causes of candidiasis. In this work, we evaluated the in
vitro susceptibility profile of C. albicans, C. parapsilosis, and C. tropicalis biofilms isolated
from patients with candidemia to fluconazole, voriconazole, amphotericin B, and caspofungin.
Differences between the biofilm ultrastructure of the three species were also determined.
The isolates were phenotypically determined by growth on a ChromagarTM medium and
assimilation profile on ID32C. The Scanning Electron Microscopy method (SEM) on biofilm
was performed using polyurethane strips. For the in vitro susceptibility profile a microdilution
in broth was used. Sessile cells were resistant to fluconazole, voriconazole and caspofungin.
The resistance to amphotericin B was less pronounced and more variable between the tested
isolates. In the SEM, slight differences in ultrastructural morphology for each species in
biofilms were observed. Our results verified biofilm formation. Low susceptibility to the drugs
in the three researched species confirmed the higher virulence of them.
Descrição
Palavras-chave
Biofilm, Candida spp., In vitro susceptibility, Scanning electron microscopy
Citação
COSTA, Carolina Rodrigues; MARRETO, Lais Carneiro Nassiazene Lima; ROCHA, Viviane Lopes; SILVA, Thaisa Cristina da; SILVA, Maria do Rosário Rodrigues. Biofilm forming capability and antifungal susceptibility profile of candida spp. from blood. Revista de Patologia Tropical, Goiânia, v. 47, n. 3, p. 159-166, jul./sep. 2018. Disponível em: https://www.revistas.ufg.br/iptsp/article/view/52351/25403.