Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium
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2017
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Calcium is considered an essential element for the metabolism of aquatic snail Biomphalaria glabrata (Say, 1818),
intermediate host of Schistosoma mansoni Sambon, 1907 in Brazil, and represents a limiting factor to its distribution
and adaptation to the environment. This study investigated the effect of different concentrations of exogenous CaCO3
on the energetic metabolism of B. glabrata for better understanding the physiological interference of chemical elements
dissolved in the environment with the physiology of this species. Sixty-day-old snails were distributed into six groups,
five exposed to different concentrations of CaCO3
(20, 40, 60, 80 and 100 mg/L) and a control group. The exposure to
CaCO3
was assessed over time, with analysis of 15 snails of each group in the following intervals: 1, 14, 21 or 30 days
for hemolymph extraction. Concentrations of calcium and glucose in the hemolymph were determined by commercial
kits, and organic acids were extracted using an ion exchange column and analyzed by high-performance liquid
chromatography. Concentration of calcium in the hemolymph showed no significant difference (p>0.05) from the
control group and between the concentrations tested. Concentration of glucose decreased (p<0.05) in the treatments
of exposure to 20 and 40 mg/L and increased when exposed to 80 and 100 mg/L CaCO3
compared to control and
to other concentrations tested over 30 days. The organic acids pyruvate, oxaloacetate, citrate, succinate, fumarate,
beta-hydroxybutyrate and lactate presented increased concentrations, while propionate and acetoacetate, decreased
concentrations, when exposed to CaCO3
compared to control. Considering the influence of different periods of exposure
to CaCO3
, on the 14th day, there were stronger alterations in the metabolism of B. glabrata. In conclusion, exposure to
CaCO3
reduced the concentration of glucose, which is metabolized into pyruvate, the final product of glycolysis, and
also influenced the energetic metabolism pathways, indicating an aerobic or partially anaerobic functioning.
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Palavras-chave
Snails, Krebs cycle, CaCO3 exposure, Energetic metabolism, Moluscos, Ciclo de Krebs, Exposição ao CaCO3, Metabolismo energético
Citação
SILVA, L. D. et al. Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium. Brazilian Journal of Biology, São Carlos, v. 77, n. 2, p. 304-311, 2017. DOI: 10.1590/1519-6984.13315. Disponível em: https://www.scielo.br/j/bjb/a/ZNMpj9xgzSZ5jfw3TRmTxvL/?lang=en. Acesso em: 21 fev. 2025.