Genomic surveillance of Neisseria meningitidis serogroup B invasive strains: diversity of vaccine antigen types, Brazil, 2016-2018
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2020
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Background
Neisseria meningitidis serogroup B remains a prominent cause of invasive meningococcal
disease (IMD) in Brazil. Because two novel protein-based vaccines against serogroup B are
available, the main purpose of this study was to provide data on the diversity and distribution
of meningococcal vaccine antigen types circulating in Brazil.
Methodology
Genetic lineages, vaccine antigen types, and allele types of antimicrobial-associated resistance genes based on whole-genome sequencing of a collection of 145 Neisseria meningitidis serogroup B invasive strains recovered in Brazil from 2016 to 2018 were collected.
Results
A total of 11 clonal complexes (ccs) were identified among the 145 isolates, four of which
were predominant, namely, cc461, cc35, cc32, and cc213, accounting for 72.0% of isolates.
The most prevalent fHbp peptides were 24 (subfamily A/variant 2), 47 (subfamily A/variant
3), 1 (subfamily B/variant 1) and 45 (subfamily A/variant 3), which were predominantly associated with cc35, cc461, cc32, and cc213, respectively. The NadA peptide was detected in
only 26.2% of the isolates. The most frequent NadA peptide 1 was found almost exclusively
in cc32. We found seven NHBA peptides that accounted for 74.5% of isolates, and the
newly described peptide 1390 was the most prevalent peptide exclusively associated with
cc461. Mutated penA alleles were detected in 56.5% of the isolates, whereas no rpoB and
gyrA mutant alleles were found.
Conclusion
During the study period, changes in the clonal structure of circulating strains were observed,
without a predominance of a single hyperinvasive lineage, indicating that an epidemiologic
shift has occurred that led to a diversity of vaccine antigen types in recent years in Brazil.
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LEMOS, Ana Paula Silva de et al. Genomic surveillance of Neisseria meningitidis serogroup B invasive strains: diversity of vaccine antigen types, Brazil, 2016-2018. Plos One, San Francisco, v. 15, n. 12, e0243375, 2020. DOI: 10.1371/journal.pone.0243375. DisponĂvel em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751880/. Acesso em: 12 ago. 2024.