Isolamento e caracterização de fungos em coleções entomológicas

Abstract

Entomological collections are taken like study sources for the understanding of mechanisms of human diseases in which the insects act, like spreading vectors. The maintenance of these collections is a permanent worry for parasitologists. Fungi contamination in these insects is frequent and the development of more efficient techniques is required through the knowledge of the fungi classes involved. Twelve boxes from the entomological collections of the Parasitology Division of the Institulc of Tropical Pathology and Public Health from the Federal University of Goiás \vere analy/ed. Each one of the 1.200 cm2 boxes held about 200 bugs, 400 mosquitoes and 300 flies. Sixty insects were chosen from these boxes at random. with presutnable mould, mildew and/or yeast assault. Fungi infection was identified in 28 (46,7%) insects from 60 analyzed insects. Twelve (42,8%) strains of Acremonium sp were identified, each one being verified in 2 samples of Culex mosquito, members of the Sarcophagidae family, Crysomyia puturia, Trialoma infestans and 4 Rhodnius neglectus. A strain of Chaetomium .\p (14,3%) was detected in one sample of Musca domestica and in 7 other samples, 2 (7,1%) strains of Aspergilius sp, 2 (7,1%) QÍ Alternaria sp and 3 (10,7%) of Curvularia sp. Five (18,0%) isolates of Penicillhim sp were noticed in 2 samples of Triatoma infestans and 3 Rhodnius neglectus, Three (10,7%) cultures of Fusarium sp were isolated only in samples of Culex mosquito. The filamentous fungi keep on being the main microorganisms involved in the assault in entomological collections.