Cell surface hydrophobicity and biofilm formation of candida yeast species in different culture media
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2017
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Resumo
Cell surface adhesion is considered an essential step in the spread, infection and persistence of
Candida yeasts in the host. Their ability to adhere on biotic and abiotic surfaces depends on several factors, including
hydrophobicity. Once attached, these yeasts are capable of growing in biofilms, which are constituted of structured
communities of encapsulated cells within an extracellular matrix, resistant to antifungal agents. In this context, this study
aimed to analyze the cell surface hydrophobicity and specific biofilm formation of six Candida strains in different culture
media: Sabouraud dextrose broth (SDB), artificial saliva (AS), Roswell Park Memorial Institute medium (RPMI 1640) and
N-acetylglucosamine-yeast nitrogen base proline (NYP). Six yeasts of the genus Candida were studied: three C. albicans
(Ca): Ca ATCC 10231 and the clinical isolates Ca34 and Ca05 and C. parapsilosis (Cp): Cp ATCC 22019 and the clinical
isolates Cp120 and Cp38. Hydrophobicity was calculated as the percentage reduction in turbidity of the aqueous phase,
due to the retention of the hydrophobic cells in hydrocarbon by the hydrocarbon-water biphasic assay following the
MATH (Microbial Adhesion to Hydrocarbon) method. The biofilm formation index was calculated as the optical density
obtained by the growth of the yeasts in the culture media in a polystyrene microtiter plate, subsequently stained with 1%
violet crystal. The results showed that hydrophobicity varied according to the media and the yeasts studied, and two of
these (Ca34 and Ca10231) presented significant variation between the media. A more hydrophobic character was observed
in yeasts grown on RPMI-1640 medium, and those grown on Sabouraud dextrose broth appeared more hydrophilic. The
specific biofilm formation index was more intense for RPMI 1640 than in other media, which was expected, due to its
ability to induce the transition between yeast-hyphae morphology, which is one of the key factors involved in the adhesion
of C. albicans on different surfaces. RPMI 1640 was the best medium for obtaining biofilm in vitro, due to its greater
hydrophobicity, which can enhance cell adhesion to the polystyrene plate, and due to its nutrient content, necessary for
complete cell growth and biofilm formation.
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Biofilm, Hydrophobicity, Candida albicans, Candida parapsilosis, Candida albicans, Biofilme, Hidrofobicidade, Candida parapsilosis
Citação
PORFÍRIO, Bruno Ezequiel Carvalho et al. Cell surface hydrophobicity and biofilm formation of candida yeast species in different culture media. Bioscience Journal, Uberlândia, v. 33, n. 3, p. 739-746, 2017. DOI: 10.14393/BJ-v33n3-34770. Disponível em: https://seer.ufu.br/index.php/biosciencejournal/article/view/34770. Acesso em: 10 fev. 2025.
10.14393/BJ-v33n3-34770