Ecto-nucleotidase activities of promastigotes from leishmania (viannia) braziliensis relates to parasite infectivity and disease clinical outcome

Página do item simplificado
dc.creatorLeite, Pauline Martins
dc.creatorSilva, Rodrigo Gomes da
dc.creatorFigueiredo, Amanda Braga de
dc.creatorSerafim, Tiago Donatelli
dc.creatorTafuri, Wagner Luiz
dc.creatorSouza, Carolina Carvalho de
dc.creatorMoura, Sandra Aparecida Lima de
dc.creatorFietto, Juliana Lopes Rangel
dc.creatorMelo, Maria Norma
dc.creatorDias, Fátima Ribeiro
dc.creatorOliveira, Milton Adriano Pelli de
dc.creatorRabello, Ana Lúcia Teles
dc.creatorAfonso, Luis Carlos Crocco
dc.date.accessioned2018-07-23T12:53:05Z
dc.date.available2018-07-23T12:53:05Z
dc.date.issued2012-10
dc.description.abstractBackground: Leishmania (Viannia) braziliensis has been associated with a broad range of clinical manifestations ranging from a simple cutaneous ulcer to destructive mucosal lesions. Factors leading to this diversity of clinical presentations are not clear, but parasite factors have lately been recognized as important in determining disease progression. Given the fact that the activity of ecto-nucleotidases correlates with parasitism and the development of infection, we evaluated the activity of these enzymes in promastigotes from 23 L. braziliensis isolates as a possible parasite-related factor that could influence the clinical outcome of the disease. Methodology/Principal Findings: Our results show that the isolates differ in their ability to hydrolyze adenine nucleotides. Furthermore, we observed a positive correlation between the time for peak of lesion development in C57BL/6J mice and enzymatic activity and clinical manifestation of the isolate. In addition, we found that L. (V.) braziliensis isolates obtained from mucosal lesions hydrolyze higher amounts of adenine nucleotides than isolates obtained from skin lesions. One isolate with high (PPS6m) and another with low (SSF) ecto-nucleotidase activity were chosen for further studies. Mice inoculated with PPS6m show delayed lesion development and present larger parasite loads than animals inoculated with the SSF isolate. In addition, PPS6m modulates the host immune response by inhibiting dendritic cell activation and NO production by activated J774 macrophages. Finally, we observed that the amastigote forms from PPS6m and SSF isolates present low enzymatic activity that does not interfere with NO production and parasite survival in macrophages. Conclusions/Significance: Our data suggest that ecto-nucleotidases present on the promastigote forms of the parasite may interfere with the establishment of the immune response with consequent impaired ability to control parasite dissemination and this may be an important factor in determining the clinical outcome of leishmaniasis.pt_BR
dc.identifier.citationLEITE, Pauline M.; GOMES, RODRIGO S.; FIGUEIREDO, Amanda B.; SERAFIM, Tiago D.; TAFURI, Wagner L.; SOUZA, Carolina C. de; MOURA, Sandra A. L. ; FIETTO, Juliana L. R.; MELO, Maria N.; RIBEIRO-DIAS, Fátima; OLIVEIRA, Milton A. P.; RABELLO, Ana; AFONSO, Luís C. C. Ecto-nucleotidase activities of promastigotes from leishmania (viannia) braziliensis relates to parasite infectivity and disease clinical outcome. PLoS Neglected Tropical Diseases, San Francisco, v. 6, n. 10, e1850, Oct. 2012.pt_BR
dc.identifier.doi10.1371/journal.pntd.0001850
dc.identifier.issn1935-2727
dc.identifier.issne- 1935-2735
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15454
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleEcto-nucleotidase activities of promastigotes from leishmania (viannia) braziliensis relates to parasite infectivity and disease clinical outcomept_BR
dc.typeArtigopt_BR

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Artigo - Pauline Martins Leite - 2012.pdf
Tamanho:
5.31 MB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IPTSP - Artigos publicados em periódicos