The mc2-CMX vaccine induces an enhanced immune response against Mycobacterium tuberculosis compared to Bacillus Calmette-Guérin but with similar lung inflammatory effects
Nenhuma Miniatura disponível
Data
2016
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Although the attenuated Mycobacterium bovis Bacillus Calmette-Guérin (BCG) vaccine has been used since
1921, tuberculosis (TB) control still proceeds at a slow pace. The main reason is the variable efficacy of BCG protec tion against TB among adults, which ranges from 0-80%. Subsequently, the mc2
-CMX vaccine was developed with
promising results. Nonetheless, this recombinant vaccine needs to be compared to the standard BCG vaccine. The
objective of this study was to evaluate the immune response induced by mc2
-CMX and compare it to the response
generated by BCG. BALB/c mice were immunised with both vaccines and challenged with Mycobacterium tubercu losis (Mtb). The immune and inflammatory responses were evaluated by ELISA, flow cytometry, and histopathology.
Mice vaccinated with mc2
-CMX and challenged with Mtb induced an increase in the IgG1 and IgG2 levels against
CMX as well as recalled specific CD4+ T-cells that produced T-helper 1 cytokines in the lungs and spleen compared
with BCG vaccinated and challenged mice. Both vaccines reduced the lung inflammatory pathology induced by the
Mtb infection. The mc2
-CMX vaccine induces a humoral and cellular response that is superior to BCG and is effi ciently recalled after challenge with Mtb, although both vaccines induced similar inflammatory reductions.
Descrição
Palavras-chave
Rrecombinant vaccine, Tuberculosis, Inflammation, Mouse
Citação
OLIVEIRA, Fábio Muniz de; TRENTINI, Monalisa Martins; JUNQUEIRA-KIPNIS, Ana Paula; KIPNIS, André. The mc2-CMX vaccine induces an enhanced immune response against Mycobacterium tuberculosis compared to Bacillus Calmette-Guérin but with similar lung inflammatory effects. Memórias do Instituto Oswaldo Cruz, Rio de Janeio, v. 111, n. 4, p. 223-231, 2016. DOI: 10.1590/0074-02760150411. Disponível em: https://www.scielo.br/j/mioc/a/tc9Z3pT4BSSNjrzkv7yzcVw/?lang=en. Acesso em: 22 ago. 2024.