Norovirus in feces and nasopharyngeal swab of children with and without acute gastroenteritis symptoms: First report of GI.5 in Brazil and GI.3 in nasopharyngeal swab
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2017
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Background: Noroviruses (NoVs) are an important cause of acute gastroenteritis (AGE), worldwide.
Objectives: To evaluate the frequency, viral load and molecular profile of NoV in fecal and nasopharyngeal
swab samples from hospitalized children, and to determine children’s secretor status.
Study design: From May 2014 to May 2015, 219 children were included in the study, 96 with gastroenteric
symptoms and 123 without gastroenteric symptoms. All fecal and nasopharyngeal swab samples were
screened by TaqMan RT-qPCR duplex (GI/GII NoV) and quality samples were characterized by genomic
sequencing.
Results: Norovirus positivity rate in feces was 15.4% in asymptomatic and 18.8% in the symptomatic
group. The median viral loads in feces were 2.69 × 108 GC/g and 4.32 × 107 GC/g from children with or
without AGE symptoms, respectively. In nasopharyngeal swab samples, the NoV positivity was 11.4% in
symptomatic children, with a median viral load of 2.20 × 107 GC/mL and 6.5% in asymptomatic children,
with an average viral load of 1.73 × 106 GC/mL. In only two cases NoV was detected in both samples. A
considerable genomic variability was observed in feces, with six genotypes being detected, as follows:
GII.4, GII.6, GI.3 and GII.3, GI.2 and GI.5. Two GI.3 was detected in nasopharyngeal swab.
Conclusions: Our data reveal considerable NoVfrequencies in both nasopharyngeal and fecal samples from
symptomatic and asymptomatic children. Higher viral loads were detected in samples from AGE symp tomatic children, when compared to asymptomatic children. High genomic variability was observed,
with this being the first report of GI.5 NoV in Brazil and of GI.3 in nasopharyngeal swab samples.
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Norovirus, Hospitalized children, Viral load NoV in nasopharyngeal swab, Genotypes, Asymptomatic NoV infection, RT-qPCR
Citação
DÁBILLA, Nathânia et al. Norovirus in feces and nasopharyngeal swab of children with and without acute gastroenteritis symptoms: first report of GI.5 in Brazil and GI.3 in nasopharyngeal swab. Journal of Clinical Virolog, Amsterdam, v. 87, p. 60-66, 2016. DOI: 10.1016/j.jcv.2016.12.009. Disponível em: https://www.sciencedirect.com/science/article/pii/S1386653216306370?via%3Dihub. Acesso em: 26 ago. 2024.