In vitro stressing factors altering the TCA cycle and morphology of Taenia crassiceps cysticerci

Vinaud, Marina Clare; Andrade, Lilian Cristina Morais de; Alves, Patricia Fernandes Melo; Fraga, Carolina Miguel; Bezerra, José Clecildo Barreto; Lino Junior, Ruy de Souza

In vitro stressing factors altering the TCA cycle and morphology of Taenia crassiceps cysticerci

Data

2016

Autores

Vinaud, Marina Clare

Andrade, Lilian Cristina Morais de

Alves, Patricia Fernandes Melo

Fraga, Carolina Miguel

Bezerra, José Clecildo Barreto

Lino Junior, Ruy de Souza

Resumo

Objective To determine the morphological and biochemical alterations of in vitro induced Taenia crassiceps cysticerci by the presence of glucose, insulin and praziquantel isolated and in association. Methods The cysticerci were cultured for 24 h in supplemented Roswell Park Memorial Institute culture medium and added to two different concentrations of glucose, insulin and praziquantel. The morphometrical analysis was performed through the ImageJ programme, and the biochemical one through high-performance liquid chromatography. Results The exposure to the stressing factors led to alterations in the morphology and decrease in the growth rate of the parasite. Conclusions The metabolic effects are related to a decrease in the tricarboxylic acid cycle and fatty acids oxidation metabolites due to the drug's mode of action. Interestingly, the praziquantel, insulin and glucose association enhanced the drug's mode of action with a greater decrease of the tricarboxylic acid cycle metabolites.

Citação

VINAUD, Marina Clare et al. In vitro stressing factors altering the TCA cycle and morphology of Taenia crassiceps cysticerci. Asian Pacific Journal of Tropical Disease, Singapore, v. 6, n. 1, p. 49-53, 2016. DOI: 10.1016/S2222-1808(15)60984-7. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S2222180815609847. Acesso em: 24 fev. 2025.