Aspectos da infecção por Anaplasma marginaleem bovinos experimentalmente infectados
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Este estudio tuvo como objetivo evaluar las características clínicas, hematológicas, aspectos
parasitológicos, así como la eficacia de oxitetraciclina de larga acción contra Anaplasma
marginale en el ganado infectado experimentalmente. Seis animales seronegativos fueron
obtenidos por RIFI. En día 9 del estudio, los tres animales fueron esplenectomizados, y de día
cero, seis animales fueron inoculados con aproximadamente 1x106
glóbulos rojos parasitados
por Anaplasma marginale. La temperatura rectal se midio desde el primero al 35 º día después
de la inoculación (DPI). Haga que el volumen celular empacado (VG - microhematocrito ) y
el cálculo de la parasitemia, fueron obtenidos a partir de la 10ª a la 35ª DPI. Los animales
recibieron un tratamiento específico (oxitetraciclina LA), cuando se presenta inicialmente la
hipertermia ( ≥ 39,2° C) y la parasitemia ≥ 2,0 %, y recibir nuevos tratamientos, clínicos y
parasitológicos hasta que se produce la estabilización del animal. Analizando los resultados,
parece que a los 21 DPI la parasitemia estaba presente en seis animales, en el mismo dia,
comenzó el declive de VG en el ganado. La temperatura rectal del ganado alcanzó fiebre ≥
39,2 picos del 23 DPI, en este día que la parasitemia de los animales se encontraban en el
lugar . En general, el tratamiento específico para A. marginale, se tuvo que realizar unos tres Los animales habían recibido uno a cuatro tratamientos específicos contra A. marginale para
la estabilización de los animales clínicos/parasitológica. Hubo total eliminación de los
parásitos después del tratamiento requiere el primer tratamiento específico para A. marginale,
aproximadamente 72 horas después de la detección directa del parásito en las cuchillas . Se
tomó entre uno y cuatro tratamientos para la recuperación clínica y reducción parasitológica
en los animales. Además, el período de pre - patente y por lo tanto la incubación de esta
enfermedad fue, en promedio, 21 días, sin embargo, este período puede extender un poco más
de acuerdo con la cantidad de inóculo usado o también la ruta de administración utilizó para
infectar el ganado.
This study aimed to evaluate the clinical, hematological, parasitological aspects and the effectiveness of long-acting oxytetracycline against Anaplasma marginale in experimentally infected cattle. Six 10-month-old animals seronegative by indirect immunofluorescence reaction (IFAT) were obtained. On day -9 of the study, three animals were splenectomized, and on day zero all six animals were inoculated with about 1x106 by Anaplasma marginale parasitized erythrocytes. Rectal temperature was measured from the 1st to the 35th day post inoculation (DPI). The packed cell volume (VG - microhematocrit) and calculation of parasitemia was obtained from 10º to 35º DPI. The experimental animals received first specific treatment (oxytetracycline LA) when they presented hyperthermia (≥39.2°C) and parasitemia ≥2.0%, and received new treatments until clinical and parasitological stabilization occurred. The results revealed that on 21º DPI parasitemia was present in the six animals, and from this day on the VG decreased. Their rectal temperature reached ≥39.2oC from 23º DPI on, when the parasitemia was on the rise. In general, the specific treatment against A. marginale had to be performed about three days after the haemoparasite was identified in the blood. The animals received one to four specific treatments against A. marginale to obtain clinical and parasitological stabilization. The parasite was not totally eliminated after the treatments. Based on the experimental model used in this study, one can conclude that the specific treatment required against A. marginale had to start about 72 hours after detection of the parasite on the cover slips. One to four treatments were required for clinical recovery of the animals and parasitological reduction of the infection. The incubation period for the disease was on average 21 days; however, this period may extend more, according to the amount of inoculum or the route of administration used to infect the cattle.
This study aimed to evaluate the clinical, hematological, parasitological aspects and the effectiveness of long-acting oxytetracycline against Anaplasma marginale in experimentally infected cattle. Six 10-month-old animals seronegative by indirect immunofluorescence reaction (IFAT) were obtained. On day -9 of the study, three animals were splenectomized, and on day zero all six animals were inoculated with about 1x106 by Anaplasma marginale parasitized erythrocytes. Rectal temperature was measured from the 1st to the 35th day post inoculation (DPI). The packed cell volume (VG - microhematocrit) and calculation of parasitemia was obtained from 10º to 35º DPI. The experimental animals received first specific treatment (oxytetracycline LA) when they presented hyperthermia (≥39.2°C) and parasitemia ≥2.0%, and received new treatments until clinical and parasitological stabilization occurred. The results revealed that on 21º DPI parasitemia was present in the six animals, and from this day on the VG decreased. Their rectal temperature reached ≥39.2oC from 23º DPI on, when the parasitemia was on the rise. In general, the specific treatment against A. marginale had to be performed about three days after the haemoparasite was identified in the blood. The animals received one to four specific treatments against A. marginale to obtain clinical and parasitological stabilization. The parasite was not totally eliminated after the treatments. Based on the experimental model used in this study, one can conclude that the specific treatment required against A. marginale had to start about 72 hours after detection of the parasite on the cover slips. One to four treatments were required for clinical recovery of the animals and parasitological reduction of the infection. The incubation period for the disease was on average 21 days; however, this period may extend more, according to the amount of inoculum or the route of administration used to infect the cattle.
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Anaplasmosis, Oxitetraciclina, Fiebre por garrapatas del ganado, Parasitemia, Anaplasmosis, Oxytetracycline, Tick fever, Parasitemia, Anaplasmose, Oxitetraciclina, Tristeza parasitária bovina, Parasitemia
Citação
LOPES, Welber Daniel Zanetti et al. Aspectos da infecção por Anaplasma marginaleem bovinos experimentalmente infectados. Veterinária e Zootecnia, Botucatu, v. 23, n. 2, p. 272-284, 2016. Disponível em: https://rvz.emnuvens.com.br/rvz/article/view/764. Acesso em: 13 ago. 2025.