Fisetin as a promising antifungal agent against Cryptocococcus neoformans species complex
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2016
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Resumo
The aim of this study was to investigate the mechanisms of action of
fisetin, a flavonol with antifungal activity previously evaluated against the
Cryptococcus neoformans species complex.
Methods and Results: Ergosterol content and flow cytometry analysis were
determined for the C. neoformans species complex in the presence of fisetin
and ultrastructural analysis of morphology was performed on Cryptococcus
gattii and C. neoformans. Decrease in the total cellular ergosterol content after
exposure to fisetin ranged from 25 4% after exposure to 128 lg ml 1 to 21 6%
after exposure to 64 lg ml 1
of fisetin compared with the control (without
fisetin). The fisetin effects obtained with flow cytometry showed metabolic
impairment, and alterations in its normal morphology caused by fisetin in
C. neoformans cells were verified using scanning electron microscopy.
Conclusions: Fisetin is a compound that acts in the biosynthesis of ergosterol.
Flow cytometry showed that fisetin reduced viability of the metabolically active
cells of C. gattii, while morphological changes explain the action of fisetin in
inhibiting growth of these fungi.
Significance and Impact of the Study: This study supports the idea that fisetin
may represent a good starting point for the development of future therapeutic
substances for cryptococcosis.
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Antifungal activity, Cryptococcus neoformans, Fisetin, Mechanisms of action, Scanning electron microscopy
Citação
REIS, M. P. C. et al. Fisetin as a promising antifungal agent against Cryptocococcus neoformans species complex. Journal of Applied Microbiology, Oxfrod, v. 121, n. 2, p. 373-379, 2016. DOI: 10.1111/jam.13155. Disponível em: https://academic.oup.com/jambio/article/121/2/373/6717309?login=true. Acesso em: 10 fev. 2025.