Blood transcriptome profile induced by an efficacious vaccine formulated with salivary antigens from cattle ticks
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2019
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Ticks cause massive damage to livestock and vaccines are one sustainable alternative for the acaricide poisons currently heavily
used to control infestations. An experimental vaccine adjuvanted with alum and composed by four recombinant salivary antigens
mined with reverse vaccinology from a transcriptome of salivary glands from Rhipicephalus microplus ticks was previously shown to
present an overall efficacy of 73.2% and cause a significant decrease of tick loads in artificially tick-infested, immunized heifers; this
decrease was accompanied by increased levels of antigen-specific IgG1 and IgG2 antibodies, which were boosted during a
challenge infestation. In order to gain insights into the systemic effects induced by the vaccine and by the tick challenge we now
report the gene expression profile of these hosts’ whole-blood leukocytes with RNA-seq followed by functional analyses. These
analyses show that vaccination induced unique responses to infestations; genes upregulated in the comparisons were enriched for
processes associated with chemotaxis, cell adhesion, T-cell responses and wound repair. Blood transcriptional modules were
enriched for activation of dendritic cells, cell cycle, phosphatidylinositol signaling, and platelets. Together, the results indicate that
by neutralizing the tick’s salivary mediators of parasitism with vaccine-induced antibodies, the bovine host is able to mount normal
homeostatic responses that hinder tick attachment and haematophagy and that the tick otherwise suppresses with its saliva.
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MARUYAMA, Sandra R. et al. Blood transcriptome profile induced by an efficacious vaccine formulated with salivary antigens from cattle ticks. NPJ Vaccines, Berlin, v. 4, e53, 2019. DOI: 10.1038/s41541-019-0145-1. Disponível em: https://www.nature.com/articles/s41541-019-0145-1#citeas. Acesso em 29 jan. 2025.