Molecular epidemiological analysis of bloodstream isolat es of Candida albicans
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Data
2008-12
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Editor
Ruy de Souza Lino Junior
Resumo
Candidemia is generally related to the endogenous flora, however exogenous infection originated
from hospital staff or from the environment has occurred. The randomly amplified polymorphic
DNA (RAPD) method can reveal strain specific variation. In this study, we used a RAPD assay
to assess genetic diversity among Candida albicans isolates to find the relatedness between DNA
patterns of the strains recovered from clinical and environment samples from the Intensive Care
Unit (ICU) in the Hospital das Clínicas of the Universidade Federal de Goiás. The primers named
Cnd3 (5´-CCAGATGCAC-3`) and Cnd4 (5’-ACGGTACACT-3`) were used as single primers in
the PCR. RAPD profiles from both blood and urine from the same patients were identical in almost
all the samples studied, except in one patient. The bed of this patient had the same genotype from
his blood. Although most of C. albicans isolates probably had had an endogenous origin, the finding
of isolates from the patients with the same profile as the environment isolates suggest that the
candidemia may have resulted from an exogenous source.
Descrição
Palavras-chave
Candida albicans, RAPD, Nosocomial infection, Infecções nosocomiais
Citação
PASSOS, Xisto Sena; SALES, Werther de Souza; COSTA, Carolina Rodrigues; LEMOS; Janine de Aquino; SOUZA, Lúcia Kioko Hasimoto e; SOUZA, Keili Maria Cardoso de; PEREIRA, Luiz Augusto; SILVA, Maria do Rosário Rodrigues. Molecular epidemiological analysis of bloodstream isolates of Candida albicans. Revista de Patologia Tropical, Goiânia, v. 37, n. 4, p. 323-331, out./dez. 2008. Disponível em: <https://www.revistas.ufg.br/iptsp/article/view/5664/4496>.