Comparative analyses of the structure of the 1,3-Beta glucan synthase gene in Paracoccidioides brasiliensis isolates
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Data
2006
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Resumo
The evolutionary origin and significance of spliceosomal
introns have been the subject of many investigations. Two theories, “in-
trons-early” theory and “introns-late” theory, have been proposed to
explain the evolution of introns in eukaryotic genes. Intron position is
generally conserved in paralogue and orthologue genes. Some introns
occur at similar but not necessarily identical positions in homologous
genes, which were separated by great evolutionary distances. This event
can be explained by insertion, loss or movement of the intron over short
distances. Intron loss and gain events are unique in evolution and can be
useful as markers for phylogenetic analyses. The insertion of introns at
an identical position suggests a common ancestor gene. Here we ana-
lyzed, using PCR and RT-PCR, the structure of the 1,3-β-glucan syn-
thase gene (FKS) in several clinical isolates of Paracoccidioides bra-
siliensis (Pb): isolates Pb 01, Pb 4940, Pb 8515, Pb 8311, Pb 8334, Pb
4268, Pb 1668, and Pb E. Our results showed that seven of the isolates
examined showed identical structures concerning the position of introns
in PbFKS1. PbFKS4940 showed the intron described at the 3’ end and had lost that one at the 5’ end. The presence of the PbFKS4940 tran-
script suggests that it could be a functional gene. These data suggest a
divergent evolution for introns with regard to the 1,3-β-glucan synthase
gene in P. brasiliensis isolates.
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Paracoccidioides brasiliensis, Gene structure, Intron evolution, 1,3-β-glucan synthase
Citação
OKAMOTO, H. T. S.; SOARES, C. M. A.; PEREIRA, M. Comparative analyses of the structure of the 1,3-Beta glucan synthase gene in Paracoccidioides brasiliensis isolates. Genetics and Molecular Research, Ribeirão Preto, v. 5, n. 2, p. 407-418, 2006.