Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross‑over trial
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2020
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In this study, we evaluated the efects of autologous serum collected after two types of exercise
on the in vitro infammatory profle and T cell phenotype of resting peripheral blood mononuclear
cells (PBMCs) in obese men. Serum samples and PBMCs were obtained from eight obese men who
performed two exercise bouts—high intensity interval exercise (HIIE) and exhaustive exercise session
to voluntary fatigue—in a randomized cross-over trial. Pre-exercise PBMCs were incubated with
50% autologous serum (collected before and after each exercise bout) for 4 h. In vitro experiments
revealed that post-HIIE serum reduced the histone H4 acetylation status and NF-κB content of
PBMCs and suppressed the production of both TNF-α and IL-6 by PBMCs, while increasing IL-10
production. Post-exhaustive exercise serum induced histone H4 hyperacetylation and mitochondrial
depolarization in lymphocytes and increased TNF-α production. In vitro post-HIIE serum incubation
resulted in an increase in the frequencies of CD4+CTLA-4 + and CD4 +CD25+ T cells expressing CD39
and CD73. Post-exhaustive exercise serum decreased the frequency of CD4+CD25 +CD73+ T cells but
increased CD4+CD25-CD39 +T cell frequency. Both post-exercise serums increased the proportions of
CD4 +PD-1 + and CD8 +PD-1+ T cells. Blood serum factors released during exercise altered the immune
response and T cell phenotype. The type of exercise impacted the immunomodulatory activity of the
post-exercise serum on PBMCs.
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DORNELES, Gilson P. et al. Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial. Scientific Reports, London, v. 10, e21735, 2020. DOI: 10.1038/s41598-020-78750-z. Disponível em: https://www.nature.com/articles/s41598-020-78750-z. Acesso em: 23 abr. 2025.