New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli

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dc.creatorXavier, Mauro Aparecido Souza
dc.creatorKipnis, Andre
dc.creatorTorres, Fernando Araripe Gonçalves
dc.creatorAstolfi Filho, Spartaco
dc.date.accessioned2021-02-11T12:28:13Z
dc.date.available2021-02-11T12:28:13Z
dc.date.issued2009
dc.description.abstractWe report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.pt_BR
dc.identifier.citationXAVIER, Mauro Aparecido Souza et al. New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 4, p. 778-781, 2009.pt_BR
dc.identifier.doi10.1590/S1517-83822009000400007 
dc.identifier.issn1517-8382
dc.identifier.issne- 1678-4405
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/19354
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHeterologous expressionpt_BR
dc.subjectInduced expression.pt_BR
dc.subjectEscherichia colipt_BR
dc.subjectMolecular cloningpt_BR
dc.titleNew vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia colipt_BR
dc.typeArtigopt_BR

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