Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test

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dc.creatorSilva, Bosco Christiano Maciel da
dc.creatorGrassi, Maria Fernanda Rios
dc.creatorCoutinho Junior, Raimundo
dc.creatorMascarenhas, Rita Elizabeth Moreira
dc.creatorOlavarria Gallazzi, Viviana Nilla
dc.creatorBorgo, Adriana Coutinho
dc.creatorKalil Filho, Jorge Elias
dc.creatorCunha Neto, Edecio
dc.creatorFonseca, Simone Gonçalves da
dc.date.accessioned2018-08-16T12:12:50Z
dc.date.available2018-08-16T12:12:50Z
dc.date.issued2014-12
dc.description.abstractThe interferon (IFN)-γ response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4 + T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-γ enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-γ production by PBMCs from at least 31% of the TST-positive donors. The magnitude of the response against all pep- tides was 182 ± 230 x 10 6 IFN-γ spot forming cells (SFC) among TST-positive donors and 36 ± 62 x 10 6 SFC among TST-negative donors (p = 0.007). The response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-γ assays to identify individuals with this condition.pt_BR
dc.identifier.citationSILVA, Bosco Christiano Maciel da; GRASSI, Maria Fernanda Rios; COUTINHO, Raimundo; MASCARENHAS, Rita Elizabeth Moreira; OLAVARRIA, Viviana Nilla; COUTINHO-BORGO, Adriana; KALIL, Jorge; CUNHA-NETO, Edecio; FONSECA, Simone Gonçalves. Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 8, p. 999-1004, Dec. 2014.pt_BR
dc.identifier.doi10.1590/0074-0276140193
dc.identifier.issn0074-0276
dc.identifier.issne- 1678-8060
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15614
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectMycobacterium tuberculosispt_BR
dc.subjectMantoux tuberculin skin testpt_BR
dc.subjectEpitopespt_BR
dc.subjectIFN-γ ELISPOTpt_BR
dc.subjectLatent tuberculosispt_BR
dc.titleMycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin testpt_BR
dc.typeArtigopt_BR

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