Profile of enterobacteria resistant to Beta-Lactams
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Data
2020
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Resumo
A serious emerging problem worldwide is increased antimicrobial resistance. Acquisition of
coding genes for evasion methods of antimicrobial drug mechanisms characterizes acquired resistance.
This phenomenon has been observed in Enterobacteriaceae family. Treatment for bacterial infections
is performed with antibiotics, of which the most used are beta-lactams. The aim of this study was to
correlate antimicrobial resistance profiles in Enterobacteriaceae by phenotypic methods and molecular
identification of 14 beta-lactamase coding genes. In this study, 70 exclusive isolates from Brazil were
used, half of which were collected in veterinary clinics or hospitals Phenotypic methodologies were
used and real-time PCR was the molecular methodology used, through the Sybr Green system.
Regargding the results found in the tests it was observed that 74.28% were resistant to ampicillin,
62.85% were resistant to amoxicillin associated with clavalunate. The mechanism of resistance that
presented the highest expression was ESBL (17.14%). The genes studied that were detected in a
greater number of species were blaGIM and blaSIM (66.66% of the samples) and the one that was
amplified in a smaller number of samples was blaVIM (16.66%). Therefore, high and worrying levels
of antimicrobial resistance have been found in enterobacteria, and a way to minimize the accelerated
emergence of their resistance includes developing or improving techniques that generate diagnoses
with high efficiency and speed.
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Antibiogram, Enterobacterias; molecular diagnosis, β-lactams, Antimicrobial resistance
Citação
SANTOS, Andressa Liberal et al. Profile of enterobacteria resistant to Beta-Lactams. Antibiotics, Basel, v. 9, e410, 2020. DOI: 10.3390/antibiotics9070410. Disponível em: https://www.mdpi.com/2079-6382/9/7/410. Acesso em: 3 jun. 2025.