Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats
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2021
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Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii because they are highly sensitive and
specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of
Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled
cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in
15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR.
The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by
Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S
marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the
molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%.
Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers
(k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was
able to detect samples considered negative by PFT.
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Toxoplasma gondii, Copro-PCR, Gatos, Cats
Citação
IGREJA, Jaqueline Ataíde Silva Lima da et al. Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats. Revista Brasileira de Parasitologia Veterinaria, São Paulo, v. 30, n. 2, e000621, 2021. DOI: 10.1590/S1984-29612021022. Disponível em: https://www.scielo.br/j/rbpv/a/ytJPpzKjzrc64fkZxkzxjyz/?lang=en. Acesso em: 30 abr. 2025.