Punicalagin triggers ergosterol biosynthesis disruption and cell cycle arrest in Cryptococcus gattii and Candida albicans

Página do item simplificado
dc.creatorSilva, Thaísa Cristina
dc.creatorMarcelino, Renato Ivan de Ávila
dc.creatorZara, Ana Laura de Sene Amâncio
dc.creatorSantos, Andressa Santana
dc.creatorAtaídes, Fábio Silvestre
dc.creatorFreitas, Vivianny Aparecida Queiroz
dc.creatorCosta, Carolina Rodrigues
dc.creatorValadares, Marize Campos
dc.creatorSilva, Maria do Rosário Rodrigues
dc.date.accessioned2025-06-06T11:51:16Z
dc.date.available2025-06-06T11:51:16Z
dc.date.issued2020
dc.description.abstractPunicalagin is a phenolic compound extracted from Lafoensia pacari A. St.-Hil (Lythraceae) leaves. It has demonstrated interesting activity against pathogenic fungi, e.g., Cryptococcus gattii and Candida albicans, by inhibiting fungi growth in a minimum inhibitory concentration (MIC) at 4 μg/mL. However, the mechanisms behind its antifungal action are not well understood. In this study, certain parameters were investigated, by transmission electron microscopy, ergosterol synthesis inhibition, and flow cytometry analyses, to gain insight into the possible biological targets of punicalagin (4 or 16 μg/mL) against yeast cells. Data showed that, in contrast to untreated cells, punicalagin triggered severe ultrastructural changes in C. gattii and C. albicans, such as disorganization of cytoplasmic content and/or thickened cell walls. In addition, it caused a decrease in yeast plasma membrane ergosterol content in a concentration-dependent manner. However, it was unable to bring about significant fungal cell membrane rupture. On the other hand, punicalagin (16 μg/mL) significantly arrested C. albicans and C. gattii cells at the G0/G1 phase, with a consequent reduction in cells at the G2/M phase in both fungi isolates, and thereby prevented progression of the normal yeast cell cycle. However, these alterations showed no involvement of reactive oxygen species overproduction in C. albicans and C. gattii cells, although punicalagin triggered a significant loss of mitochondrial membrane potential in C. albicans. These findings suggest that punicalagin is a promising plant-derived compound for use in developing new antifungal therapies.
dc.identifier.citationSILVA, Thaísa Cristina et al. Punicalagin triggers ergosterol biosynthesis disruption and cell cycle arrest in Cryptococcus gattii and Candida albicans. Brazilian Journal of Microbiology, Rio de Janeiro, v. 51, n. 4, p. 1719-1727, 2020. DOI: 10.1007/s42770-020-00364-4. Disponível em: https://link.springer.com/article/10.1007/s42770-020-00364-4. Acesso em: 5 jun. 2025.
dc.identifier.doi10.1007/s42770-020-00364-4
dc.identifier.issn1517-8382
dc.identifier.issne- 1678-4405
dc.identifier.urihttps://link.springer.com/article/10.1007/s42770-020-00364-4
dc.language.isoeng
dc.publisher.countryBrasil
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Restrito
dc.titlePunicalagin triggers ergosterol biosynthesis disruption and cell cycle arrest in Cryptococcus gattii and Candida albicans
dc.typeArtigo

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IPTSP - Artigos publicados em periódicos