Detection of human papillomavirus and the role of p16INK4a in colorectal carcinomas
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2020
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Introduction
Human papillomavirus (HPV) infection is associated with the development of anogenital and
head and neck cancers. In recent years a potential role of HPV in colorectal cancer (CRC)
has been suggested.
Objective
To investigate the presence of HPV in colorectal carcinomas and to study the role of
p16INK4a as a marker of transcriptionally active HPV infection. In addition, to investigate the
correlation between these findings and the CRC prognostic factors.
Methods
Case control study with 92 cases of colorectal cancers, 75 controls of normal tissue adjacent
to the tumor, and 30 controls of precursor lesions, including polyps and colorectal adeno mas. Paraffinized samples were used, HPV detection and genotyping were performed by
PCR and reverse hybridization by using the INNO LIPA kit, with SPF10 plus primers. The
expression of the p16INK4a protein was investigated using immunohistochemistry. Data
analysis was performed using descriptive, univariate statistics and survival curves were cal culated by using the Kaplan Meier and log-rank method.
Results
HPV was detected in 13% of the cases and the most prevalent genotype was HPV 16. HPV
DNA was not detected in either control groups. The high expression of p16INK4a was
observed in 30% of the cases, but it was not associated to the presence of HPV. The overall
survival was 53.3% and was influenced by prognostic factors such as later stage, lymph
node and distant metastasis.
Conclusions
Based on these results, HPV is unlikely to be involved in colorectal carcinogenesis and
p16INK4a expression is not a relevant marker of transcriptionally active HPV infection in
CRC.
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LIBERA, Larisse Silva Dalla et al. Detection of human papillomavirus and the role of p16INK4a in colorectal carcinomas. Plos One, San Francisco, v. 15, n. 6, e0235065, 2020. DOI: 10.1371/journal.pone.0235065. Disponível em: https://pmc.ncbi.nlm.nih.gov/articles/PMC7316293/. Acesso em: 6 mar. 2025.