Molecular identifi cation and antifungal susceptibility profi les of Candida parapsilosis complex species isolated from culture collection of clinical samples

Página do item simplificado
dc.creatorAtaídes, Fábio Silvestre
dc.creatorCosta, Carolina Rodrigues
dc.creatorSouza, Lúcia Kioko Hasimoto e
dc.creatorFernandes, Orionalda de Fátima Lisboa
dc.creatorJesuino, Rosalia Santos Amorim
dc.creatorSilva, Maria do Rosário Rodrigues
dc.date.accessioned2018-06-29T12:23:47Z
dc.date.available2018-06-29T12:23:47Z
dc.date.issued2015-08
dc.description.abstractCandida parapsilosis is a common yeast species found in cases of onychomycosis and candidemia associated with infected intravascular devices. In this study, we differentiated Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis from a culture collection containing blood and subungual scraping samples. Furthermore, we assessed the in vitro antifungal susceptibility of these species to fl uconazole, itraconazole, voriconazole, posaconazole, amphotericin B, and caspofungin. Methods: Differentiation of C. parapsilosis complex species was performed by amplifi cation of the secondary alcohol dehydrogenase (SADH) gene and digestion by the restriction enzyme BanI. All isolates were evaluated for the determination of minimal inhibitory concentrations using Etest, a method for antifungal susceptibility testing. Results: Among the 87 isolates, 78 (89.7%) were identifi ed as C. parapsilosis sensu stricto, fi ve (5.7%) were identifi ed as C. orthopsilosis, and four (4.6%) were identifi ed as C. metapsilosis. Analysis of antifungal susceptibility showed that C. parapsilosis sensu stricto isolates were less susceptible to amphotericin B and itraconazole. One C. parapsilosis sensu stricto isolate was resistant to amphotericin B and itraconazole. Moreover, 10.2% of C. parapsilosis sensu stricto isolates were resistant to caspofungin. Two C. parapsilosis sensu stricto isolates and one C. metapsilosis isolate were susceptible to fl uconazole in a dose-dependent manner. Conclusions: We reported the fi rst molecular identifi cation of C. parapsilosis complex species in State of Goiás, Brazil. Additionally, we showed that although the three species exhibited differences in antifungal susceptibility profi les, the primary susceptibility of this species was to caspofungin.pt_BR
dc.identifier.citationATAIDES, Fábio Silvestre; COSTA, Carolina Rodrigues; SOUZA, Lúcia Kioko Hasimoto; FERNANDES, Orionalda Defátima Lisboa; JESUINO, Rosália Santos Amorim; SILVA, Maria do Rosário Rodrigues. Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 48, n. 4, p. 454-459, July/Aug. 2015.pt_BR
dc.identifier.doi10.1590/0037-8682-0120-2015
dc.identifier.issn0037-8682
dc.identifier.issne- 1678-9849
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15333
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectCandida parapsilosispt_BR
dc.subjectOnychomycosispt_BR
dc.subjectIn vitro antifungal susceptibilitypt_BR
dc.subjectCandidemiapt_BR
dc.titleMolecular identifi cation and antifungal susceptibility profi les of Candida parapsilosis complex species isolated from culture collection of clinical samplespt_BR
dc.typeArtigopt_BR

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Artigo - Fábio Silvestre Ataides - 2015.pdf
Tamanho:
910.18 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IPTSP - Artigos publicados em periódicos