Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells

Página do item simplificado
dc.creatorDias, Fátima Ribeiro
dc.creatorNascimento, F. R. F.
dc.creatorBarbuto, José Alexandre Marzagão
dc.creatorTimenetsky, Jorge
dc.creatorNegro, Sonia Jancar
dc.date.accessioned2018-07-19T15:54:56Z
dc.date.available2018-07-19T15:54:56Z
dc.date.issued1998
dc.description.abstractMacrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elic- ited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited mac- rophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%) and low production of NO (4.7 ± 3.1 μM NO 2- ). These macrophages co-cultured with mycoplasma-in- fected YAC-1 cells showed significantly higher cytotoxic activity (61.4 ± 9.1%; P<0.05) and higher NO production (48.5 ± 13 μM NO 2- ; P<0.05). Addition of L-NAME (10 mM), an inhibitor of NO synthesis, to these co-cultures reduced the cytotoxic activity to 37.4 ± 2% (P<0.05) and NO production to 3 ± 4 μM NO 2- (P<0.05). The present data show that Mycoplasma arginini is able to induce macro- phage cytotoxic activity and that this activity is partially mediated by NO.pt_BR
dc.identifier.citationRIBEIRO-DIAS, F.; RUSSO, M.; NASCIMENTO, F. R. F. ; BARBUTO, J. A. M. ; TIMENETSKY, J.; JANCAR, S . Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 31, n. 11, p. 1425-1428, Nov. 1998.pt_BR
dc.identifier.doi10.1590/S0100-879X1998001100010
dc.identifier.issn0100-879X
dc.identifier.issne- 1414-431X
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15430
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectMacrophagespt_BR
dc.subjectCytotoxicitypt_BR
dc.subjectMycoplasmapt_BR
dc.subjectTumor cellspt_BR
dc.subjectNitric oxidept_BR
dc.titleThioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cellspt_BR
dc.typeArtigopt_BR

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Artigo - Fátima Rieiro Dias - 1998.pdf
Tamanho:
36.75 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IPTSP - Artigos publicados em periódicos