Development of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1

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dc.creatorJapolla, Greice
dc.creatorCunha Junior, Jair Pereira da
dc.creatorPajuaba, Ana Cláudia Arantes Marquez
dc.creatorTaketomi, Ernesto Akio
dc.creatorBührer, Samira
dc.creatorBataus, Luiz Artur Mendes
dc.creatorSouza, Guilherme Rocha Lino de
dc.date.accessioned2025-04-15T14:39:11Z
dc.date.available2025-04-15T14:39:11Z
dc.date.issued2018
dc.description.abstractBovine herpesvirus type 1 (BoHV-1) is recognized as an important pathogen causing respiratory, reproductive, and neurological disorders in cattle and is associated with economic losses to animal industry. Accurate diagnostic methods are needed for prevention of disease transmission. While the virus neutralization test is considered the gold standard method, it requires maintenance of the virus and cell cultures, which is time consuming and expensive. Serological techniques such as enzyme-linked immunosorbent assay (ELISA) are widely applied, as these are easy to perform and provide quick results. In the present study, a nanogold slot blot inhibition assay was developed for the serological diagnosis of BoHV-1 and compared with standard ELISA and horseradish peroxidase (HRP) slot blot assays. Of 42 serum samples tested by ELISA, 32 (76.2%) were positive and 10 (23.8%), were negative. The sensitivity and specificity of the nanogold slot blot inhibition assay was similar to that observed for ELISA and HRP slot blot assays, and a strong correlation was observed between the tests. Thus, the nanogold slot blot inhibition assay may serve as an efficient and rapid alternative to ELISA in settings, where plate-reading equipment is lacking.
dc.identifier.citationJAPOLLA, Greice et al. Development of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1. Archives of Virology, New York, v. 163, n. 3, p. 1549-1557, 2018. DOI: 10.1007/s00705-018-3763-4. Disponível em: https://link.springer.com/article/10.1007/s00705-018-3763-4. Acesso em: 11 abr. 2025.
dc.identifier.doi10.1007/s00705-018-3763-4
dc.identifier.issn0304-8608
dc.identifier.issne- 1432-8798
dc.identifier.urihttps://link.springer.com/article/10.1007/s00705-018-3763-4
dc.language.isoeng
dc.publisher.countryEstados unidos
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Restrito
dc.titleDevelopment of a nanogold slot blot inhibition assay for the detection of antibodies against bovine herpesvirus type 1
dc.typeArtigo

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