Pimenta pseudocaryophyllus inhibits virulence factors and promotes metabolic changes in Candida yeast

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dc.creatorEl Assal, Flávio Ezzeddine
dc.creatorPaula, Joelma Abadia Marciano
dc.creatorSilva, Leonardo Capeletti da
dc.creatorAbrão, Fernando Yano
dc.creatorAtaídes, Fábio Silvestre
dc.creatorSá, Fabyola Amaral da Silva
dc.creatorCosta, Carolina Rodrigues
dc.creatorFernandes, Orionalda de Fátima Lisboa
dc.creatorSouza, Lúcia Kioko Hasimoto e
dc.creatorSilva, Maria do Rosário Rodrigues
dc.date.accessioned2018-06-29T15:03:27Z
dc.date.available2018-06-29T15:03:27Z
dc.date.issued2014-10
dc.description.abstractThis is the fi rst study to examine the in vitro susceptibility and the expression of virulence factors in Candida species in the presence of Pimenta pseudocaryophyllus (Gomes) L.R. Landrum (Myrtaceae), a Brazilian plant known as pau- cravo. Additionally, the mechanisms of action of the crude ethanol extract and the ethyl acetate and aqueous fractions of this plant were investigated. Methods: The in vitro susceptibility of Candida was tested using the broth microdilution method, whereas an XTT reduction assay was used for biofi lms. Adherence was determined by counting the number of yeast cells that adhered to 100 oral epithelial cells, and hyphal formation was verifi ed in the hyphal induction medium M199. Flow cytometry with propidium iodide and FUN-1 was performed to assess the mechanism of action. Results: The results revealed that the crude ethanol extract and the ethyl acetate and aqueous fractions of P. pseudocaryophyllus inhibited the growth of Candida isolates at a minimal inhibitory concentration (MIC) ranging from 64 to 256μg/mL, whereas the 50% sessile minimal inhibitory concentration (SMIC 50 ) ranged from 512 to >1,024μg/mL. Adherence and hyphal formation were signifi cantly reduced in the presence of the crude ethanol extract and both fractions. Although cell membrane injury was detected, the predominant mechanism of action appeared to be the alteration of yeast metabolism, as demonstrated by fl ow cytometry. Conclusions: Our results indicated that antifungal activity reduced the expression of virulence factors in yeast via the alteration of yeast metabolism, suggesting that the crude extract of P. pseudocaryophyllus and its fractions may contain novel antifungal agents.pt_BR
dc.identifier.citationEL ASSAL, Flávio Ezeddinne; PAULA, Joelma Abadia Marciano; CAPELETTI, Leonardo Silva; ABRÃO, Fernando Yano; ATAÍDES, Fábio Silvestre; SÁ, Fabyola Amaral da Silva; COSTA, Carolina Rodrigues; FERNANDES, Orionalda de Fátima Lisboa; SOUZA, Lúcia Kioko Hasimoto; SILVA, Maria do Rosario Rodrigues. Pimenta pseudocaryophyllus inhibits virulence factors and promotes metabolic changes in Candida yeast. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 47, n. 5, p. 618-623, Sept./Oct. 2014.pt_BR
dc.identifier.doi10.1590/0037-8682-0135-2014
dc.identifier.issn0037-8682
dc.identifier.issne- 1678-9849
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15336
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectPimenta pseudocaryophylluspt_BR
dc.subjectMetabolic changespt_BR
dc.subjectCandida spp.pt_BR
dc.subjectAntifungal activitypt_BR
dc.subjectVirulence factorspt_BR
dc.titlePimenta pseudocaryophyllus inhibits virulence factors and promotes metabolic changes in Candida yeastpt_BR
dc.typeArtigopt_BR

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