Validação do teste sorológico para toxoplasmose em papel filtro
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Data
2019
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Resumo
Objective: To validate the use of the serological test for toxoplasmosis
in filter paper in blood samples from pregnant women from the city of
Goiânia and the metropolitan region. Methods: This is a prospective
study in which 1,006 blood samples were collected in pregnant women
in the city of Goiânia and in the metropolitan region. All pregnant women
who agreed to participate in the study signed a Free and Informed Consent
Form. Serological profile evaluation was performed using ELISA
(immunoenzymatic assay), with serum and filter paper samples, to
investigate anti-Toxoplasma gondii antibodies of the IgM and IgG clas ses, and the kit used is not standardized for the research of IgM antibodies
on the filter paper. Statistical analysis was performed in the database of
the EpiInfo® version 3.2.1 program, which evaluated the prevalence of
seropositivity, frequency of serum positivity, filter paper positivity frequency,
sensitivity, specificity, positive predictive value, negative predictive value
and index the kappa. Results: In the serum analysis, 421 samples were
positive for antibodies of the IgG class and three were positive for
antibodies of the IgM class. In the filter paper yielded 443 positive for
antibodies of the IgG class and one positive for IgM class antibodies.
Conclusion: The study allowed to identify the similarity of positive
samples both with the use of serum and in the filter paper for IgG class
antibodies. However, for serum IgM antibodies, the use of serum was
more sensitive when compared to serum IgG. taken with the filter paper.
Descrição
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Diagnóstico, Sorologia, Gestantes, Toxoplasmose, Diagnosis, Serology, Pregnant women, Toxoplasmosis
Citação
OLIVEIRA, Sarah Ribeiro de et al. Validação do teste sorológico para toxoplasmose em papel filtro. Revista Brasileira de Análises Clínicas, Rio de Janeiro, v. 51, n 1, p. 76-80, 2019. DOI: 10.21877/2448-3877.201900801. Disponível em: https://www.rbac.org.br/revista-rbac/1-2/. Acesso em: 30 abr. 2025.