Correlation between intestinal BMP2, IFNγ, and neural death in experimental infection with Trypanosoma cruzi
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2021
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Megacolon is one of the main late complications of Chagas disease, affecting approximately
10% of symptomatic patients. However, studies are needed to understand the mechanisms
involved in the progression of this condition. During infection by Trypanosoma cruzi (T.
cruzi), an inflammatory profile sets in that is involved in neural death, and this destruction is
known to be essential for megacolon progression. One of the proteins related to the mainte nance of intestinal neurons is the type 2 bone morphogenetic protein (BMP2). Intestinal
BMP2 homeostasis is directly involved in the maintenance of organ function. Thus, the aim
of this study was to correlate the production of intestinal BMP2 with immunopathological
changes in C57Bl/6 mice infected with the T. cruzi Y strain in the acute and chronic phases.
The mice were infected with 1000 blood trypomastigote forms. After euthanasia, the colon
was collected, divided into two fragments, and a half was used for histological analysis and
the other half for BMP2, IFNγ, TNF-α, and IL-10 quantification. The infection induced
increased intestinal IFNγ and BMP2 production during the acute phase as well as an
increase in the inflammatory infiltrate. In contrast, a decreased number of neurons in the
myenteric plexus were observed during this phase. Collagen deposition increased gradually
throughout the infection, as demonstrated in the chronic phase. Additionally, a BMP2
increase during the acute phase was positively correlated with intestinal IFNγ. In the
same analyzed period, BMP2 and IFNγ showed negative correlations with the number of
neurons in the myenteric plexus. As the first report of BMP2 alteration after infection by
T. cruzi, we suggest that this imbalance is not only related to neuronal damage but may also
represent a new route for maintaining the intestinal proinflammatory profile during the acute
phase.
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CARMO NETO, José Rodrigues do et al. Correlation between intestinal BMP2, IFNγ, and neural death in experimental infection with Trypanosoma cruzi. Plos One, San Francisco, v. 16, n. 2, e0246692, 2021. DOI: 10.1371/journal.pone.0246692. Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246692. Acesso em: 5 fev. 2025.