Phenotype responses to abiotic stresses, asexual reproduction and virulence among isolates of the entomopathogenic fungus Cordyceps javanica (Hypocreales: Cordycipitaceae)
Nenhuma Miniatura disponível
Data
2018
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Selecting entomopathogenic fungal isolates with resilience to environmental stresses, optimal mass production
characteristics, and with high virulence to target pests favors the development of mycopesticides. A case in point,
Cordyceps (= Isaria) javanica has been extensively investigated for non-chemical control of whiteflies worldwide.
We phylogenetically characterized 11 native C. javanica isolates from Northeastern and Central Brazil. These
isolates were screened for tolerance to heat-shock, UV-B radiation, osmotic and oxidative stresses, as well as
conidial production on cereal grain and insecticidal activity against the whitefly Bemisia tabaci (MEAM 1) in the
laboratory. All isolates were pathogenic to whiteflies and significant (3-fold) differences in median lethal con centration were observed among isolates. Furthermore, pronounced differences among isolates were found for
stress factors and conidial production. Using principal component analysis, our results highlighted three major
clusters formed by isolates (i) resistant to osmotic and oxidative stress, (ii) resilient to UV-B, and (iii) with high
virulence, conidial production and heat tolerance. Overall, isolate CG1228 performed best based on multi-stress
resistance, mass production and virulence attributes in the laboratory. This study highlights the importance of
exploring natural variation in entomopathogenic fungi for selection of appropriate isolates for effective bio control of insect pests coupled with mass production characteristics and abiotic stress tolerances.
Descrição
Palavras-chave
Biological control, UV-B tolerance, Heat-Shock, Oxidative stress, Osmotic stress, Principal component analysis
Citação
MASCARIN, Gabriel Moura et al. Phenotype responses to abiotic stresses, asexual reproduction and virulence among isolates of the entomopathogenic fungus Cordyceps javanica (Hypocreales: Cordycipitaceae). Microbiological Research, v. 216, p. 12-22, 2018. DOI: 10.1016/j.micres.2018.08.002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0944501318306815?via%3Dihub. Acesso em: 12 dez. 2024.