Improving peptide quantification in chitosan nanoparticles
Nenhuma Miniatura disponível
Data
2018
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The objective of the present study was to evaluate different methodologies for peptide quantification in the su pernatant of chitosan nanoparticles by removing the unliked polymer in the suspension. The ionic gelation
method was used to prepare the chitosan nanoparticles encapsulating a 5.3 kDa peptide. Three different meth odologies for the processment of the solutions were compared before subjecting the samples to the Bradford pro tocol or Qubit® kit for protein detection. For the quantification, it was necessary to create a standard peptide
curve using different peptide concentrations. The suitable standard curve would be one in which the peptide
was diluted in the empty chitosan supernatant (obtained after nanoparticles centrifugation) or in the filtrate of
the empty chitosan supernatant. Our results indicated the safest methodology tested for peptide quantification
in the supernatant chitosan nanoparticles was filtering the solution before subjecting the sample to the Bradford
assay
Descrição
Palavras-chave
Chitosan, Biomolecules quantification, Nanoparticle
Citação
RIBEIRO, Maycon Carvalho et al. Improving peptide quantification in chitosan nanoparticles. International Journal of Biological Macromolecules, Guildford, v. 119, p. 32-36, 2018. DOI: 10.1016/j.ijbiomac.2018.07.119. Disponível em: https://www.sciencedirect.com/science/article/pii/S0141813018331167?via%3Dihub. Acesso em: 8 maio 2025.