Dendritic cell line AP284 supports Th17 amplification
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Data
2019
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Resumo
Dendritic cells (DC) have the unique ability to capture microorganisms and activate naive T lymphocytes.
Obtaining DC derived from progenitors demands high cost and prolonged cultivation. Different immortalized DC
has been isolated but most of them have immature phenotype and depending on growing factors or other stimuli
to be used. In this study we characterized the cell line AP284 as a DC. AP284 cells express high levels of CD11b,
MHC class II, 33D1 and CD209b. They also express high amounts of CD80 costimulatory molecule and different
toll like receptors (TLR). After stimuli with TLR agonist they produce surprising amount of IL-12p40 related to
IL-23 formation but not IL-12p70. They are also able to produce IL-6 and favor amplification of a Th17 but not
Th1 profile. This DC line may be useful for a better understanding of factors and cellular interactions responsible
for the induction of IL-12p40, IL-23 and Th17 generation.
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Immortalized dendritic cell, IL-12p40, IL-23 Th17, Toll like receptor
Citação
OLIVEIRA, Pollyana Guimarães de et al. Dendritic cell line AP284 supports Th17 amplification. Cellular Immunology, New York, v. 337, p. 54-61, 2019. DOI: 10.1016/j.cellimm.2019.02.003. Disponível em: https://www.sciencedirect.com/science/article/pii/S0008874918304684?via%3Dihub. Acesso em: 20 mar. 2025.