Isolamento, identificação e avaliação das atividades enzimática e antibacteriana de micro-organismos endofíticos de Hyptis suaveolens (L.) Poit

Abstract

Endophytic are microorganisms that spent part or all the life cycle inside vegetal tissues. Under stress conditions, as hydric deficit, phytopathogen invasion or contact with toxic substances, they generate diverse biomolecules that aid in the vegetal protection. The actual study aimed to isolate and evaluate the enzymatic and antimicrobial potential of endophytic microorganisms from Hyptis suaveolens (L.) Poit., a vegetal species broadly applied in the Brazilian phytotherapy. A total of 39 microbial isolates were selected from different parts of the plants. After the identification, it was found the most part of the isolated microorganisms were bacteria belonging to the genera Pseudomonas sp. (23,1%), Bacillus sp. (23,0%), Streptomyces sp. (20.5%), Alcaligenes sp. (17,9%), Micrococcus sp. (5,1%), Comamonas sp. (2,6%), Acinetobacter sp. (2,6%) and Staphylococcus sp. (2,6%), and a yeast from the genus Rhodotorula sp. (2.6%). At least one type of a exoenzyme action (amylase, lipase, esterase, pectinase or protease) was observed for 25 isolates (64,1%). Concerning the in vitro screening of the antimicrobial activity, it was observed 15 isolates (38,5%) exhibited capacity to inhibit the growth of some of the 16 target microorganisms, especially the strains Pseudomonas aeruginosa MC 7B and Pseudomonas fluorescens MC 15, which presented the highest level of antibiotic action against 15 (93,8%) and 9 (56,2%) of the indicator bacterial strains, respectively. In conclusion, the study highlights the endophytic community from this medicinal plant exhibits a profitable potencial for the generation of enzymes of industrial interest and antimicrobials.