Murine macrophages do not support the proliferation of Leishmania (Viannia) braziliensis amastigotes even in absence of nitric oxide and presence of high arginase activity

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dc.creatorTeixeira, Mirian Vieira
dc.creatorSoares, Santiago Aguiar Espellet
dc.creatorSouza, Vagniton Amelio de
dc.creatorMarques, André Murilo de Souza
dc.creatorSoares, Célia Maria de Almeida
dc.creatorBaeza, Lilian Cristiane
dc.creatorOliveira, Milton Adriano Pelli
dc.date.accessioned2025-03-24T10:22:38Z
dc.date.available2025-03-24T10:22:38Z
dc.date.issued2022
dc.description.abstractLeishmania (Viannia) braziliensis is the main species responsible for American tegumentary leishmaniasis in Brazil. Nevertheless, the use of this parasite species to study Leishmania infection in the murine model has been less conducted when compared with other Leishmania species. The control of murine infection with Leishmania has been associated with nitric oxide (NO) produced by inducible NO synthase (iNOS) from M1 macrophages, while arginase expressed by M2 macrophages is related to Leishmania proliferation. Here we use three different strains of L. (V.) braziliensis and one strain of L. (L.) major to study a 9-day infection of macrophages in vitro. Wild-type bone marrow–derived macrophages (BMDM) supported the proliferation of L. (L) major amastigotes from the 3rd day after infection, while all strains of L. (V.) braziliensis did not proliferate even inside IL-4-treated or iNOS knockout (KO) macrophages. The arginase activity was higher in iNOS KO than IL-4-treated macrophage showing that the absence of proliferation is independent of arginase. Importantly, L. (V.) braziliensis was able to cause uncontrolled disease in iNOS KO mice in vivo demonstrating that murine macrophages present at the site of infection have additional changes beyond inhibition of NO production or stimulation of arginase activity to support parasite proliferation.
dc.identifier.citationTEIXEIRA, Mirian Vieira et al. Murine macrophages do not support the proliferation of Leishmania (Viannia) braziliensis amastigotes even in absence of nitric oxide and presence of high arginase activity. Parasitology Research, Berlin, v. 121, n. 10, p. 2891-2899, 2022. DOI: 10.1007/s00436-022-07614-4. Disponível em: https://link.springer.com/article/10.1007/s00436-022-07614-4. Acesso em 19 mar. 2025.
dc.identifier.doi10.1007/s00436-022-07614-4
dc.identifier.issn0932-0113
dc.identifier.issne- 1432-1955
dc.identifier.urihttps://link.springer.com/article/10.1007/s00436-022-07614-4
dc.language.isoeng
dc.publisher.countryAlemanha
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Restrito
dc.titleMurine macrophages do not support the proliferation of Leishmania (Viannia) braziliensis amastigotes even in absence of nitric oxide and presence of high arginase activity
dc.typeArtigo

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