Antigen-specific secretion of IFNγ and CXCL10 in whole blood assay detects Mycobacterium leprae infection but does not discriminate asymptomatic infection from symptomatic leprosy

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dc.creatorPinto, Emerith Mayra Hungria
dc.creatorFreitas, Aline de Araújo
dc.creatorPontes, Maria Araci de Andrade
dc.creatorGonçalves, Heitor Sá
dc.creatorSousa, Ana Lúcia Osório Maroccolo de
dc.creatorCosta, Maurício Barcelos
dc.creatorCastilho, Mirian Lane de Oliveira Rodrigues
dc.creatorDuthie, Malcolm S.
dc.creatorStefani, Mariane Martins de Araújo
dc.date.accessioned2025-02-17T14:32:50Z
dc.date.available2025-02-17T14:32:50Z
dc.date.issued2017
dc.description.abstractTo advance toward a whole blood assay (WBA)-based test capable of facilitating the diagnosis of paucibacillary (PB) leprosy, we evaluated a prototype in-tube WBA using combinations of Mycobacterium leprae antigens. Blood was collected from newly diagnosed untreated PB (n = 38), multibacillary (MB) (n = 30), healthy household contacts (HHC) of MB (n = 27), and endemic controls (n = 61) residing in Goiânia and Fortaleza, Brazil. Blood was incubated with M. leprae cell sonicate, recombinant proteins (46f + LID-1; ML0276 + LID-1), or controls (phosphate-buffered saline, phytohemagglutinin, M. tuberculosis purified protein derivative). Antigen-specific IFNγ production was observed in 71–84% and 55% of PB and HHC, respectively. Antigen-specific CXCL10 levels were similarly assessed to determine if, unlike IFNγ, CXCL10 could differentiate PB from HHC with repeated exposure/asymptomatic M. leprae infection. The CXCL10 levels induced in response to M. leprae antigens could not, however, differentiate PB from HHC. Despite these limitations, the WBAs reported here still represent important tools for assessing M. leprae infection rates and evaluating the impact of control measures.
dc.identifier.citationHUNGRIA, Emerith Mayra et al. Antigen-specific secretion of IFNγ and CXCL10 in whole blood assay detects Mycobacterium leprae infection but does not discriminate asymptomatic infection from symptomatic leprosy. Diagnostic Microbiology and Infectious Disease, New York, v. 87, n. 4, p. 328-334, 2017. DOI: 10.1016/j.diagmicrobio.2017.01.002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0732889317300068?via%3Dihub. Acesso em: 12 fev. 2025.
dc.identifier.doi10.1016/j.diagmicrobio.2017.01.002
dc.identifier.issn0732-8893
dc.identifier.issne- 1879-0070
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0732889317300068?via%3Dihub
dc.language.isoeng
dc.publisher.countryEstados unidos
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Restrito
dc.subjectLeprosy
dc.subjectDiagnosis
dc.subjectCell-mediated immunity
dc.subjectWhole blood assay
dc.subjectIFNγ
dc.subjectCXCL10
dc.titleAntigen-specific secretion of IFNγ and CXCL10 in whole blood assay detects Mycobacterium leprae infection but does not discriminate asymptomatic infection from symptomatic leprosy
dc.typeArtigo

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