Mycobacterium abscessus cell wall and plasma membrane characterization by EPR spectroscopy and effects of amphotericin B, miltefosine and nerolidol
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2022
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Spin label electron paramagnetic resonance (EPR) spectroscopy was used to characterize the components of the
Mycobacterium abscessus massiliense cell envelope and their interactions with amphotericin B (AmB), miltefosine
(MIL), and nerolidol (NER). Spin labels analogous to stearic acid and phosphatidylcholine (PC) were distributed
on an envelope layer with fluidity comparable to other biological membranes, probably the mycobacterial cell
wall, because after treatment with AmB a highly rigid spectral component was evident in the EPR spectra. Methyl
stearate analogue spin labels found a much more fluid membrane and did not detect the presence of AmB, except
for at very high drug concentrations. Unlike other spin-labeled PCs, the TEMPO-PC spin probe, with the nitroxide
moiety attached to the choline of the PC headgroup, also did not detect the presence of AmB. On the other hand,
the steroid spin labels were not distributed across the membranes of M. abscessus and, instead, were concentrated
in some other location of the cell envelope. Both MIL and NER compounds at 10 μM caused increased fluidity in
the cell wall and plasma membrane. Furthermore, NER was shown to have a remarkable ability to extract lipids
from the mycobacterial cell wall. The EPR results suggest that the resistance of mycobacteria to the action of
AmB must be related to the fact that this drug does not reach the bacterial plasma membrane.
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Mycobacterium abscessus, Amphotericin B, Miltefosine, Nerolidol, Spin label, Electron paramagnetic resonance
Citação
ALONSO, Lais; PIMENTA, Laryssa Ketelyn Lima; KIPNIS, André; ALONSO, Antonio. Mycobacterium abscessus cell wall and plasma membrane characterization by EPR spectroscopy and effects of amphotericin B, miltefosine and nerolidol. Biochimica et Biophysica Acta (BBA) - Biomembranes, Amsterdam, v. 1864, n. 5, e183872, 2022. DOI: 10.1016/j.bbamem.2022.183872.
1879-2642. Disponível em: https://www.sciencedirect.com/science/article/pii/S0005273622000141. Acesso em: 26 ago. 2024.