Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara)
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Data
2004
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Resumo
Fluorine is an element much used by man and easily found in nature.
Some studies have been carried out on the toxicity of fluorine and its
cumulative effect on animal tissues. Sodium fluoride is described as
being used in controlling the host of schistosomiasis (Biomphalaria
glabrata). With a view to preserve the aquatic environment, it is
proposed to verify the effect of sodium fluoride on fish (Poecilia vivipara). Ten fishes were divided into two groups. The first group
was exposed to freshwater with sodium fluoride with a concentration
of 5 ppm for a period of 21 days; the second group was exposed to
freshwater. After 21 days the animals were sacrificed and their gills
removed and processed according to histochemical/histologic
methods and ultrastructural study. The gill leaflets were diaphanized
in xylol. Histologic analyses using hematoxyline-eosin identified
branchial epithelium and revealed mucous and chloride cells. The
histochemical methods to detect glycoconjugate contents of mucous
cells used: P.A.S, P.A.S. + salivar amylase; P.A.S. + acetylation ; P.A.S.
+ reversible acetylation; Alcian blue pH 2.5; Alcian blue pH 0.5; Alcian
blue pH 2.5 + methylation; and Alcian blue pH 2.5 + reversible
methylation. An increase in secretion of mucus and an alteration to
the content of the granules were also observed, suggesting behavior
changes of mucous type to enable these animals to adapt to the
environment, thus altering the concentration of sodium fluoride.
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Palavras-chave
Brânquias, Fluoreto de sódio, Células do cloro, Esquistossomose, Células mucosas, Chloride cells, Fluorine, Schistosomiasis, Mucous cells, Gills
Citação
BRESEGUELLO, Leandro; CARDOSO, Márcia Pereira; BORGES-DE-OLIVEIRA, Rodinelli; COSTA, Marcelo Ferreira da; VARRETO, José Clecildo Bezerra; SABÓIA-MORAIS, Simone Maria Teixiera de; YAMADA, Áureo Tatsumi. Effects of sodium fluoride in gills epitelium of Guppy fish (Poecilia vivipara). Brazilian Journal of Veterinary Research and Animal Science, São Paulo, v. 41, n. 4, p. 274-280, jul./ago. 2004.