Antimicrobiana de bactérias endofíticas isoladas de Jacaranda decurrens Cham. (Carobinha-do-Campo)

Resumo

Bacteriocins are antibiotics classified as natural products, in other words, secondary metabolites produced by bacteria. Endophytic, micro-organisms that have plants as hosts in at least one life stage, are a good example of bacteriocin producers. The aim of this work was to evaluate the antimicrobial activity probably due to bacteriocin production by endophytic bacteria isolated from Jacarranda decurrens Cham. against different indicator strains and the bioactive metabolites sensibility to temperature (75- 80ºC) and to trypsin in two different media. It was used 10 endophytic bacterial isolates previously isolated from Jacaranda decurrens Cham. 70% of the isolated presented antimicrobial activity, evidenced by inhibition halos in agar plates against at least one of the 15 indicators strains. The antimicrobial compounds generated by the isolates CAR 4, CAR 5 and CAR 7 were thermolabile and resistant to trypsin. The metabolites produced by CAR 6 were sensible to the heat and the enzymatic treatment and the isolates CAR 2, CAR 4, CAR 5 and CAR 7 also produced substances sensible to these conditions. The production of the antimicrobial compounds just happened in media 523 and it was not observed the presence of bacteriophages. Substances with bacteriocinogenic activity and with broad spectrum of action against pathogenic organisms was produced by the endophytic bacteria from the medicinal plant, thus exhibiting interesting potential to be applied as antimicrobials and in biological control.

Descrição

Palavras-chave

Bactérias, Endofíticos, Potencial bacteriocinogênico, Bacteria, Endophytic, Bacteriocinogenic potential

Citação

CARRIM, Aysha et al. Antimicrobiana de bactérias endofíticas isoladas de Jacaranda decurrens Cham. (Carobinha-do-Campo). Enciclopedia Biosfera, [S. l.], v. 13, n. 23, 2016. DOI: 10.18677/Enciclopedia_Biosfera_2016_122. Disponível em: https://conhecer.org.br/ojs/index.php/biosfera/article/view/1302. Acesso em: 22 nov. 2024.