Evaluation of a commercial real-time PCR kit for detection of dengue virus in samples collected during an outbreak in Goiania, central Brazil, in 2005

dc.creatorLevi, José Eduardo
dc.creatorTateno, Adriana Fumie
dc.creatorMachado, Adriana Freire
dc.creatorRamalho, Débora Camillo
dc.creatorSouza, Vanda Akico Ueda Fick de
dc.creatorGuilarde, Adriana Oliveira
dc.creatorFeres, Valéria Christina de Rezende
dc.creatorMartelli, Celina Maria Turchi
dc.creatorTurchi, Marília Dalva
dc.creatorSiqueira Júnior, João Bosco
dc.creatorPannuti, Cláudio Sérgio
dc.date.accessioned2019-01-11T11:11:19Z
dc.date.available2019-01-11T11:11:19Z
dc.date.issued2007-06
dc.description.abstractIn the past 2 decades, dengue has reemerged in Brazil as a significant public health problem. Clinicians demand a diagnostic test with high sensitivity that is applicable during the early symptomatic phase. We aimed to test two distinct molecular methods on samples from suspected dengue cases during an outbreak in Central Brazil. Acute-phase serum specimens from 254 patients suspected of having dengue were collected during 2005 in the city of Goiaˆnia, Central Brazil. Samples were blindly evaluated by real-time and multiplex PCR in addition to routine immunoglobulin M serology and virus culture. Overall, acute dengue was confirmed by serology, multiplex PCR, or virus isolation for 80% of patients (203/254). Another four patients presented real-time PCR-positive results as the unique marker of dengue. Higher real-time PCR positivity levels and viral loads were observed in the early symptomatic phase of disease (<5 days) than after this period. Multiplex and real-time PCR assays presented a high kappa agreement (0.85). According to multiplex PCR, 60 samples harbored dengue virus type 3 (DEN-3), 4 samples harbored DEN-2, and 1 sample displayed a pattern compatible with a double infection with DEN-2 and -3. The dengue virus real-time kit was found to be practical and adjustable for high throughput, to display the best performance in the early symptomatic phase of dengue cases, and to be valuable for confirming dengue diagnosis in a timely manner.pt_BR
dc.identifier.citationLEVI, José Eduardo et al. Evaluation of a commercial real-time PCR kit for detection of dengue virus in samples collected during an outbreak in Goiania, central Brazil, in 2005. Journal of Clinical Microbiology, Washington, v. 45, n. 6, p. 1893-1897, 2007.pt_BR
dc.identifier.doi10.1128/JCM.00065-07
dc.identifier.issn0095-1137
dc.identifier.issne- 1098-660X
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16711
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleEvaluation of a commercial real-time PCR kit for detection of dengue virus in samples collected during an outbreak in Goiania, central Brazil, in 2005pt_BR
dc.typeArtigopt_BR

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