Antibacterial activity of the skin secretion of Phyllomedusa azurea (Anura: Hylidae) from the Central Brazil Cerrado

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dc.creatorAlmeida, Daniela Andrade de
dc.creatorDietz, Jefferson do Carmo
dc.creatorOliveira, Bruno Francesco Rodrigues de
dc.creatorVieira, José Daniel Gonçalves
dc.creatorMagalhães, Marta Regina
dc.creatorJesuíno, Rosália Santos Amorim
dc.date.accessioned2025-03-13T19:03:11Z
dc.date.available2025-03-13T19:03:11Z
dc.date.issued2019-04
dc.description.abstractPhyllomedusa azurea is a frog species well distributed geographically in South America, including Brazilian biomes as Pantanal and Cerrado. Compared with other anurans from the Phyllomedusinae family, there are few reports on the bioactive potential of skin-derived molecules from this species. In this perspective, the aim of the present study was to evaluate the in vitro antibacterial activity of skin secretion of P. azureaby detection of Minimum Inhibitory Concentration (MIC) of the growth of bacterial indicator strains and to determine if occurs a changing in the bacterial cell envelope permeability. The MIC determination was carried out by the microdilution plate method. The absorbance was measured and analyzed statistically using the t-test to compare two groups (0.05 % of significance). The impact of the crude extract on cell envelope permeability of Staphylococcus aureus ATCC 25923 was conducted by the crystal violet assay, and the absorbance was measured spectrophotometry followed by the calculation of the crystal violet uptake percentage. The specific MIC for S. aureus ATCC 25923 and Escherichia coli ATCC 25922 was 31.25 μg/mL, while for Bacillus subtilisATCC 6633 was 125 μg/mL and Pseudomonas aeruginosa ATCC 27853 was 250 μg/mL. The treatment with crescent concentrations of frog skin secretion increased the crystal violet uptake by S. aureus ATCC 25923 cells, suggesting an action on the cell plasma membrane. The results demonstrated that the skin secretion of P. azureapresents antibacterial activity and merit further investigations to characterize the bioactive molecules.
dc.identifier.citationALMEIDA, Daniela Andrade de et al. Antibacterial activity of the skin secretion of Phyllomedusa azurea (Anura: Hylidae) from the Central Brazil Cerrado. Revista de Biología Tropical, San Pedro, v. 67, n. 1, p. 1–10, 2019. DOI: 10.15517/rbt.v67i1.33280. Disponível em: https://revistas.ucr.ac.cr/index.php/rbt/article/view/33280. Acesso em: 13 nov. 2024.
dc.identifier.doi10.15517/rbt.v67i1.33280
dc.identifier.issn0034-7744
dc.identifier.issne- 2215-2075
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/26963
dc.language.isoeng
dc.publisher.countryOutros
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAmphibian
dc.subjectAntibacterial activity
dc.subjectPhyllomedusa azurea
dc.subjectSkin secretion
dc.titleAntibacterial activity of the skin secretion of Phyllomedusa azurea (Anura: Hylidae) from the Central Brazil Cerrado
dc.typeArtigo

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