Molecular characterization of the NSP4 gene of human group A rotavirus samples from the west central region of Brazil

Página do item simplificado
dc.creatorMiranda, Talissa de Moraes Tavares
dc.creatorBrito, Wilia Marta Elsner Diederichsen de
dc.creatorFiaccadori, Fabíola Souza
dc.creatorFreitas, Erika Regina Leal de
dc.creatorRocha, Juliana Alves Parente
dc.creatorCosta, Paulo Sérgio Sucasas da
dc.creatorGiugliano, Loreny Gimenes
dc.creatorAndreasi, Márcia Sueli Assis
dc.creatorSoares, Célia Maria Almeida
dc.creatorCardoso, Divina das Dôres de Paula
dc.date.accessioned2018-07-05T11:21:19Z
dc.date.available2018-07-05T11:21:19Z
dc.date.issued2008-05
dc.description.abstractNonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavi- rus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveil- lance of rotavirus infections and vaccine development.pt_BR
dc.identifier.citationTAVARES, Talissa de Moraes; BRITO, Wilia Marta Elsner Diederichsen de; FIACCADORI, Fabíola Souza; FREITAS, Erika Regina Leal de; PARENTE, Juliana Alves ; COSTA, Paulo Sérgio Sucasas da; GUIGLIANO, Loreny Gimenes; ANDREASI, Márcia Sueli Assis; SOARES, Célia Maria Almeida; CARDOSO, Divina das Dores de Paula. Molecular characterization of the NSP4 gene of human group A rotavirus samples from the west central region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 3, p. 288-294, May 2008.pt_BR
dc.identifier.doi10.1590/S0074-02762008000300011
dc.identifier.issn0074-0276
dc.identifier.issne- 1678-8060
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15345
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectGroup A rotaviruspt_BR
dc.subjectWest central regionpt_BR
dc.subjectNSP4 genept_BR
dc.subjectGenotypespt_BR
dc.subjectBrazilpt_BR
dc.titleMolecular characterization of the NSP4 gene of human group A rotavirus samples from the west central region of Brazilpt_BR
dc.typeArtigopt_BR

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Artigo - Talissa de Moraes Tavares Miranda - 2008.pdf
Tamanho:
980.72 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

IPTSP - Artigos publicados em periódicos