Antimicrobial and chemotactic activity of scorpion-derived peptide, ToAP2, against Mycobacterium massiliensis
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Data
2019
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Mycobacterium massiliense is a rapid growing, multidrug-resistant, non-tuberculous
mycobacteria that is responsible for a wide spectrum of skin and soft tissue infections, as well as other
organs, such as the lungs. Antimicrobial peptides had been described as broad-spectrum antimicrobial,
chemotactic, and immunomodulator molecules. In this study we evaluated an antimicrobial
peptide derived from scorpion Tityus obscurus as an anti-mycobacterial agent in vitro and in vivo.
Bioinformatics analyses demonstrated that the peptide ToAP2 have a conserved region similar
to several membrane proteins, as well as mouse cathelicidin. ToAP2 inhibited the growth of
four M. massiliense strains (GO01, GO06, GO08, and CRM0020) at a minimal bactericidal concentration
(MBC) of 200 µM. MBC concentration used to treat infected macrophages was able to inhibit 50%
of the bacterial growth of all strains. ToAP2 treatment of infected mice with bacilli reduced the
bacterial load in the liver, lung, and spleen, similarly to clarithromycin levels (90%). ToAP2 alone
recruited monocytes (F4/80low Gr1), neutrophils (F4/80− Gr1), and eosinophils (F4/80+ Gr1+).
ToAP2, together with M. massiliense infection, was able to increase F4/80low and reduce the percentage
of F4/80high macrophages when compared with infected and untreated mice. ToAP2 has in vitro
anti-microbial activity that is improved in vivo due to chemotactic activity.
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Antimicrobial peptide, Mycobacteria inhibition, Mycobacterium, Monocyte, Neutrophil
Citação
MARQUES-NETO, Lázaro M. et al. Antimicrobial and chemotactic activity of scorpion-derived peptide, ToAP2, against Mycobacterium massiliensis. Toxins, Basel, v. 10, n. 6, e219, 2018. DOI: 10.3390/toxins10060219. Disponível em: https://pubmed.ncbi.nlm.nih.gov/29848960/. Acesso em: 20 ago. 2024.